THERMAL-CYCLING PIPETTE TIP

1 . A pipette tip for aspiration and thermal-cycling of a biological sample, the pipette tip comprising:

a sample inlet port;

a pipettor interface;

multiple sample chambers, wherein the sample chambers are configured to fit into recesses of a thermal cycling block; and

a sample distribution network, wherein the sample distribution network connects the sample inlet port to the pipettor interface.

2 . The pipette tip of claim 1 , wherein the sample distribution network connects the sample inlet port to each sample chamber via an inlet channel.

3 . The pipette tip of claim 2 , wherein the inlet channel is of uniform size.

4 . The pipette tip of claim 2 , wherein the inlet channel is of variable size.

5 . The pipette tip of claim 4 , wherein the inlet channel comprises a main channel and a branching channel.

6 . The pipette tip of claim 5 , wherein the main channel decreases in size.

7 . The pipette tip of claim 2 , wherein the sample distribution network connects the pipettor interface with each sample chamber via an aspiration channel.

8 . The pipette tip of claim 7 , wherein each aspiration channel comprising at least one of a direct connection and a branched connection.

9 . The pipette tip of claim 1 , wherein the sample chambers comprise a wax layer compartment.

10 . The pipette tip of claim 1 , further comprising multiple sample ports, multiple pipettor interfaces and an array of sample chambers.

11 . The pipette tip of claim 1 , further comprising an optical layer comprising of detection-compatible material, wherein the detection-compatible material aligns with the multiple sample chambers.

12 . A system for thermal cycling of a biological sample, the system comprising:

a pipette tip, the pipette tip comprising:

a sample inlet port;

a pipettor interface;

an optical surface, the optical surface comprising of detection-compatible material;

multiple sample chambers; and

a sample distribution network,

wherein the sample distribution network connects the sample inlet port to the pipettor interface, and

wherein the detection-compatible material aligns with the multiple sample chambers; and

a thermal-cycling instrument, the instrument comprising:

a thermal-cycling block, the block comprising multiple recesses configured to fit the sample chambers;

a lid, wherein the lid comprises sealing contacts configured to align with the sample distribution network and optical openings configured to align with the sample chambers; and

an optical detector configured to align with the optical openings, the detection-compatible material, and the sample chambers.

13 . The system of claim 12 , wherein the sample distribution network comprises an inlet channel and an aspiration channel for each sample chamber.

14 . The system of claim 13 , wherein the lid provides pressure to form a seal in the inlet channel and the aspiration channel.

15 . The system of claim 13 , wherein the lid provides heat to form a seal in the inlet channel and the aspiration channel.

16 . The system of claim 14 , wherein the lid provides heat during the thermal cycling of the biological sample.

17 . The system of claim 12 , wherein the optical surface further comprises multiple lenses aligned to the sample chambers.

18 . The system of claim 12 , wherein the instrument further comprises a pipettor configured to connect to the pipettor interface.

19 . The system of claim 18 , wherein the pipette tip further comprises multiple sample ports, multiple pipettor interfaces, and an array of sample chambers.

20 . The system of claim 12 , wherein the sample chambers further comprise a wax layer compartment.

21 . A system for thermal cycling of a biological sample, the system comprising:

means for storing reagents in a plurality of sample chambers;

means for distributing the sample to the plurality of sample chambers;

means for sealing each of the sample chambers;

means for thermally cycling the biological sample; and

means for detecting the biological sample.