Method for treating small volumes with electrical current
View Patent ↗Disclosed is a method for treating biological material via electrical current, in which the biological material is added to a small volume of a buffer solution having relative high ionic strength. A strong electrical field is generated in the buffer solution by a high voltage pulse having a preset duration. The biological material is added to at most 50 μl of a buffer solution with an ionic strength of at least 100 mmol/l. By at least one voltage pulse having a preset duration of at least 10 μs, an electrical field with a field strength of at least 1 kV/cm is generated in the buffer solution. The voltage pulse is hereby interrupted at least once for a duration of at least 100 μs and is then again continued.
1. A method for treating biological material by means of an electrical current comprising
providing no more than about 50 μl of a buffer solution having an ionic strength of at least about 100 mmol/l,
adding the biological material to said buffer solution,
applying, for a preset duration of at least about 10 μs, at least one voltage pulse to said buffer solution to generate an electrical field having a field strength of at least about 1 kV/cm, wherein the voltage pulse is interrupted at least once for a duration of at least about 100 μs and is subsequently continued.
2. The method of claim 1 , wherein said voltage pulse is interrupted twice to ten times.
3. The method of claim 1 or 2 , wherein a duration of about 200 μs to about 2 ms is preset for at least one interruption of said voltage pulse.
4. The method of claim 3 , wherein the duration is about 300 μs, about 400 μs, about 500 μs, about 600 μs, about 700 μs, about 800 μs, about 900 μs, about 1 ms or about 1.5 ms.
5. The method of claim 1 , wherein the buffer solution including the biological material has a total volume of between about 1 and about 50 μl.
6. The method of claim 5 , wherein said volume is between about 10 and about 40 μl, between about 15 and about 25 μl or between about 10 to about 20 μl.
7. The method of claim 1 , wherein said voltage pulse generates an electrical field with a field strength having maximally about 10 kV/cm.
8. The method of claim 7 , wherein the field strength is about 1 to about 8 kV/cm, about 2 to about 6 kV/cm or about 2 to about 4 kV/cm.
9. The method of claim 1 , wherein the voltage pulse has a preset duration having a maximum of about 5 ms.
10. The method of claim 9 , wherein said preset duration is about 20 μs to about 2 ms, about 100 to about 1000 μs or about 100 to about 600 μs.
11. The method of claim 1 , wherein the voltage pulse is interrupted after a voltage interval of about 5 μs, about 10 μs, about 20 μs, about 30 μs, about 40 μs, about 50 μs, about 60 μs, about 100 μs or about 200 μs.
12. The method of claim 1 , wherein the electrical field is generated between two electrodes and wherein the distance between the two electrodes is about 0.5 to about 5 mm.
13. The method of claim 12 , wherein said distance is about 1 to about 4 mm or about 1.5 to about 2 mm.
14. The method of claim 1 , wherein the biological material is treated in a reaction container, which has a substantially square, rectangular or round cross-section.
15. The method of claim 9 , wherein the reaction container has a substantially rectangular reaction chamber, which is delimited laterally by two electrodes having a plane-parallel configuration.