Portable light generation and detection system
View Patent ↗A field deployable optical assembly for use in testing a light-responsive sample is disclosed. The assembly includes a microfluidic device, a first optical package, and a second optical package. The first optical package includes a light emitting diode (LED), a first optical device, and a first light-path control, the first optical package configured to guide and focus light from the LED onto the sample. The microfluidic device includes a tethered control substance. In response to a substance within the sample being associated with, and attaching to, the tethered control, the sample emits light. The second optical package includes a photo sensor, a second optical device, and a second light-path control, the second optical package configured to guide and focus the light emitted from the sample onto the photo sensor.
1. A field deployable optical assembly for use in testing a light-responsive sample, the assembly comprising:
a microfluidic device, the microfluidic device comprising a tethered control substance;
a first optical package comprising a light emitting diode (LED), a first optical device, a first light-path control, and a first reference line disposed in the center of the first light-path control, defined by the orientation of the first light-path control;
a second optical package comprising a photo sensor, a second optical device, a second light-path control, and a second reference line disposed in the center of the second light-path control, defined by the orientation of the second light-path control; and
an optical mount;
wherein the first optical package is configured to guide and focus light from the LED onto the sample;
wherein in response to a substance within the sample being associated with the tethered control and attaching to the tethered control, the sample emits light;
wherein the second optical package is configured to guide and focus the light emitted from the sample onto the photo sensor;
wherein the first optical package and the second optical package are mounted within the optical mount on the same side of, and at an angle relative to, the microfluidic device; and
wherein each reference line creating a 30 degree angle relative to the microfluidic device, with a 120 degree included angle therebetween.
2. The assembly of claim 1 , wherein:
the first light-path control and the second light-path control are configured and disposed to reduce direct transmission of light from the LED to the photo sensor.
3. The assembly of claim 1 , wherein:
the first optical device and the second optical device each comprise one of the following: a filter; a lens; and, a filter-lens combination;
the first optical device and the second optical device each comprise one of the following: glass; quartz; polycarbonate; and, a polymer material;
the first light-path control and the second light-path control each have one of the following shapes: a cylinder; a pentagonal prism; a triangular prism; and, a rectangular prism;
the first light-path control and the second light-path control each comprise one of the following reflective materials: aluminum; copper; silver; gold; and, stainless steel;
the photo sensor comprises one of the following: a Silicon photodiode; a Silicon Carbide photodiode; a positive intrinsic negative photodiode; an avalanche photodiode; a complementary metal-oxide semiconductor device; a charge-coupled device; and a photomultiplier tube;
the lens is one of the following: a spherical lens; a cylindrical lens; and, a coated Fresnel lens; and
the LED comprises a Group III-V Nitride LED.
4. The assembly of claim 1 , wherein at least one of the first optical device and the second optical device are coated with a thin film filter.
5. The assembly of claim 1 , wherein:
the microfluidic device comprises a microchannel.
6. The assembly of claim 1 , wherein:
the microfluidic device comprises a hybridization chamber.
7. The assembly of claim 1 , wherein:
the total mass of the assembly is less than 7.25 Kilograms (kg).
8. The assembly of claim 7 , wherein:
the total mass of the assembly is less than 5.5 kg.
9. The assembly of claim 8 , wherein:
the total mass of the assembly is less than 3.75 kg.
10. The assembly of claim 1 , wherein:
the first light-path control and the second light-path control are each less than 1 cubic centimeter in volume.
11. The assembly of claim 1 , wherein the angle is a fixed angle.
12. The assembly of claim 1 , wherein the angle is an adjustable angle.
13. The assembly of claim 1 , wherein:
the microfluidic device is separable from the assembly and disposable.
14. The assembly of claim 1 , wherein:
the field deployable assembly is configured to allow data communication with an external device.
15. The assembly of claim 14 , wherein:
the field deployable assembly is configured to allow data communication with a personal digital assistant (PDA).
16. The assembly of claim 1 , wherein:
the microfluidic device comprises a microarray apparatus.
17. The assembly of claim 1 , further comprising:
a heater disposed and configured to confirm the identity of the sample substance attached to the tethered control.
18. The assembly of claim 1 , wherein:
the first optical device is configured to filter out wavelengths of light emitted from the sample.
19. The assembly of claim 1 , wherein:
the second optical device is configured to filter out wavelengths of light emitted from the LED.
20. The assembly of claim 1 , wherein:
the second light-path control comprises surfaces that taper inward at a distance from the photo sensor.