Cellulose synthase promoter and method for modifying cellulose and lignin biosynthesis in plants
View Patent ↗This invention relates to an isolated cellulose synthase promoter, methods for genetically altering cellulose and lignin biosynthesis, and to methods for improving strength properties of juvenile wood and fiber in trees. The invention further relates to methods for identifying regulatory elements in a cellulose synthase promoter and to methods for augmenting expression of polynucleotides operably linked to a cellulose synthase promoter.
1. An isolated cellulose synthase promoter comprising nucleotides from position 1 to position 840 of SEQ ID NO:3.
2. A polynucleotide comprising the promoter of claim 1 , operatively linked to a polynucleotide encoding a cellulose synthase or a polynucleotide encoding a UDP-glucose binding domain.
3. A vector comprising the promoter of claim 1 .
4. The vector of claim 3 , wherein the cellulose synthase promoter is operatively linked to a polynucleotide encoding a cellulose synthase or a polynucleotide encoding a UDP-glucose binding domain.
5. A transgenic plant cell comprising the promoter of claim 1 .
6. The plant cell of claim 5 , wherein the plant cell is a tree cell.
7. The promoter of claim 1 , wherein the promoter is a stress-inducible promoter.
8. A method of inducing expression of a polynucleotide in a plant comprising the polynucleotide operatively linked to the cellulose synthase promoter of claim 1 , the method comprising exposing the plant to mechanical stress thereby inducing expression of the polynucleotide.
9. The method of claim 8 , wherein the polynucleotide encodes a cellulose synthase, and wherein expression of the polynucleotide alters a characteristic of a plant selected from the group consisting of growth, cellulose content, lignin content, juvenile wood strength, fiber strength, and a combination thereof, as compared to a control plant.
10. The method of claim 9 , wherein the altered characteristic is selected from the group consisting of accelerated growth, increased cellulose content, decreased lignin content, improved juvenile wood strength improved juvenile fiber strength, and a combination thereof.
11. A transformed plant produced by the method of claim 8 .
12. The plant of claim 11 , wherein the plant is a tree.
13. A method of altering a characteristic of a plant cell comprising the promoter of claim 1 operatively linked to a polynucleotide encoding a polypeptide, wherein the promoter comprises a negative mechanical stress response element or a positive mechanical stress response element, the method comprising exposing the plant cell to a stress to alter expression of the polypeptide, thereby altering the characteristic of the plant cell, as compared to a control plant cell.
14. A transformed plant cell produced by the method of claim 13 .
15. The method of claim 13 , wherein the promoter comprises a negative mechanical stress response element and the polypeptide comprises a cellulose synthase, and wherein the altered characteristic is selected from the group consisting of accelerated growth, increased cellulose content, decreased lignin content, improved strength of juvenile wood or fiber, and combinations thereof.
16. A transformed plant cell produced by the method of claim 15 .
17. The plant cell of claim 16 , which is from a tree.
18. The method of claim 13 , wherein the promoter comprises a positive mechanical stress response element and the polypeptide comprises a cellulose synthase, and wherein the altered characteristic is selected from the group consisting of decreased cellulose content, increased lignin content, and combinations thereof.
19. A tranformed plant cell produced by the method of claim 18 .
20. The plant cell of claim 19 , which is from a tree.
21. A transformed plant comprising the plant cell of claim 19 .
22. The plant of claim 21 , wherein the plant is a tree.