Analysis, secure access to, and transmission of array images
View Patent ↗Systems and methods are provided the autocentering, autofocusing, acquiring, decoding, aligning, analyzing and exchanging among various parties, images, where the images are of arrays of signals associated with ligand-receptor interactions, and more particularly, ligand-receptor interactions where a multitude of receptors are associated with microparticles or microbeads. The beads are encoded to indicate the identity of the receptor attached, and therefore, an assay image and a decoding image are aligned to effect the decoding. The images or data extracted from such images can be exchanged between de-centralized assay locations and a centralized location where the data are analyzed to indicate assay results. Access to data can be restricted to authorized parties in possession of certain coding information, so as to preserve confidentiality.
1. A method of encrypting the identity of a type of receptor, among multiple receptor types, which is attached to particular encoded carriers in an array of encoded carriers, comprising:
encoding the carriers using a multi-component code, wherein the multi-component code is an integer representation of the configuration of a multiple constituent planar array of L encoded carriers, such that each carrier is represented by a 4-tuple of integers having the form (l, r, g, b), wherein:
l is the position index, wherein 1≦l≦L; and
r, g, and b are integer representations of intensities of fluorescence signals (IR, IG, IB); and
uniquely associating a type of receptor with carriers encoded with a particular code.
2. The method of claim 1 wherein the code is a multi-color code.
3. The method of claim 1 wherein the receptor is an oligonucleotide or a protein.
4. The method of claim 2 wherein the colors are formed by mixing differing ratios of more than one color.
5. The method of claim 2 wherein the code is formed by combinations of more than one color.
6. The method of claim 1 wherein the carriers are beads.