Use of untranslated region of osmotin gene to enhance transgene expression in plants
View Patent ↗The present invention provides methods, vectors and gene constructs for enhancing expression of a recombinant nucleic acid sequence in transgenic plants and plant tissues. According to the present invention, nucleic acid sequences are obtained and/or derived from the 5′ and 3′ untranslated regions of genes encoding osmotin proteins and engineered to flank respective portions of a selected coding region of a vector. The vector construct may be introduced into plants and/or plant tissues through conventional procedures, resulting in enhanced expression of the selected coding region. In a preferred embodiment, the selected coding region is a chimeric gene or gene fragment expressing one or more proteins known to impart a level of insecticidal activity to a transgenic plant and/or plant tissue.
1. A method for recombinantly producing a peptide or protein comprising:
functionally linking a heterologous promoter and a nucleic acid sequence consisting of an isolated osmotin UTR element selected from the group consisting of SEQ. ID No. 1 and SEQ. ID No. 2 with a structural gene of interest.
2. A method of increasing expression of a gene in a plant cell comprising:
functionally linking at least heterologous promoter and one nucleic acid sequence consisting of an isolated osmotin UTR element selected from the group consisting of SEQ. ID No. 1 and SEQ. ID No. 2 with a structural gene of interest to create a nucleic acid construct; transforming the plant cell with the nucleic acid construct; and growing the transformed cell under conditions in which the structural gene of interest is expressed.
3. The method of claim 1 , wherein the at least one structural gene of interest comprises a gene capable of conferring a non-native phenotype in a plant.
4. The method of claim 1 , wherein the at least one structural gene of interest comprises a gene capable of conferring insecticide or herbicide resistance in a plant.
5. The method of claim 1 , wherein the at least one structural gene of interest comprises SEQ. ID. No. 5.
6. The method of claim 2 , wherein the at least one structural gene of interest comprises a gene capable of conferring a non-native phenotype in a plant.
7. The method of claim 2 , wherein the at least one structural gene of interest comprises a gene capable of conferring insecticide or herbicide resistance in a plant.
8. The method of claim 2 , wherein the at least one structural gene of interest comprises SEQ. ID. No. 5.