Systems and methods for detecting and analyzing polymers
View Patent ↗A detection system and methods for improving the ability of the detection system to recognize labels that are disposed on a polymer. Embodiments of the invention include schemes for selecting emitters and labels used within the system in a manner that allows an increase in the number of distinct labels that can be used together in a system. In other embodiments, the detection system and methods are directed to identifying portions of a detection signal that may be associated with extra labels residing within a detection zone. In other embodiments, the detection system and methods relate to using wide field imaging detectors while reducing out of focus noise contributions to detection signals of the system. Still, other embodiments relate to the use of linear array detectors to detect labels.
1. A detection system for detecting a first and second distinct labels on a polymer, the detection system comprising:
a detection zone adapted to receive the polymer for detection;
an emitter for exciting each of the first and second distinct labels on the polymer when in the detection zone, causing each of the first and second distinct labels to emit a first and second emission signal, respectively;
a minor adapted to substantially separate the first and second emission signals from one another; and
a wide field detector adapted to receive the first and second emission signals on spatially separate portions of a detection surface.
2. A method of detecting a first and second distinct label on a polymer, the method comprising:
providing a detection zone;
placing the polymer and the label into the detection zone;
emitting an excitation signal for exciting the first and second distinct labels, causing the first and second labels to emit a first and second emission signal, respectively;
substantially separating the first and second emission signals from one another; and
detecting the first and second emission signals on spatially separated portions of a detector.