Adipose tissue-derived adult stem cells for the repair of articular cartilage fractures and uses thereof
The invention provides cells, methods and compositions based upon the use of adipose tissue-derived adult stem cells in the repair of articular cartilage fractures or defects. The invention is useful in providing a treatment of articular cartilage fractures in a clinical setting.
1 . A composition comprising an isolated adipose tissue-derived adult stem cell that can differentiate into a chondrocyte in combination with a viscous, biocompatible liquid material implanted into a host, wherein the host is in need of articular cartilage repair.
2 . The composition of claim 1 , wherein the biocompatible liquid is capable of gelling at body temperature.
3 . The composition of claim 2 , wherein the biocompatible liquid is selected from the group consisting of alginate, collagen, fibrin, hyaline, or plasma.
4 . The composition of claim 1 in combination with a malleable, three dimensional matrix capable of filling an irregular cartilage defect.
5 . The composition of claim 4 , wherein the matrix is selected from the group consisting of polyglycolic-polylactic acid, poly-glycolic acid, poly-lactic acid, or suture-like material.
6 . A composition comprising an isolated adipose tissue-derived adult stem cell that can differentiate into a chondrocyte and implanted into host combined with a malleable, three dimensional matrix capable of filling an irregular cartilage defect and a solid phase, biocompatible material of sufficient structural integrity to serve as an anchor within the cancellous bone underlying the articular cartilage defect.
7 . The composition of claim 6 , wherein the biocompatible material comprises an isolated adipose tissue-derived stem cell that can differentiate into an osteoblast.
8 . The composition of claim 7 , wherein the isolated adipose tissue-derived stem cell is grown in a matrix in vitro prior to implantation.
9 . The composition of claim 1 , wherein the cell is human.
10 . The composition of claim 1 , wherein the cell is modified with a nucleic acid.
11 . A method of treating an articular cartilage defect in a host comprising implanting an isolated adipose tissue-derived adult stem cell that can differentiate into a chondrocyte.
12 . The method of claim 11 , wherein the isolated adipose tissue-derived adult stem cell is in combination with a viscous, biocompatible liquid material.
13 . The method of claim 12 , wherein the biocompatible liquid is capable of gelling at body temperature.
14 . The method of claim 13 , wherein the biocompatible liquid is selected from the group consisting of alginate, collagen, fibrin, hyaline, or plasma.
15 . The method of claim 13 further comprising a malleable, three dimensional matrix capable of filling an irregular cartilage defect.
16 . The method of claim 15 , wherein the matrix is selected from the group consisting of polyglycolic-polylactic acid, poly-glycolic acid, poly-lactic acid, or suture-like material.
17 . A method of treating an articular cartilage defect in a host comprising implanting a composition comprising an isolated adipose tissue-derived adult stem cell that can differentiate into a chondrocyte, a malleable, three dimensional matrix capable of filling an irregular cartilage defect and a solid phase, biocompatible material of sufficient structural integrity to serve as an anchor within the cancellous bone underlying the articular cartilage defect.
18 . The method of claim 17 wherein the biocompatible material comprises an isolated adipose tissue-derived stem cell that can differentiate into an osteoblast.
19 . The method of claim 18 wherein the isolated adipose tissue-derived stem cell that can differentiate into an osteoblast is grown in an matrix in vitro prior to implantation.