Device for the carrying out of chemical or biological reactions
View Patent ↗The invention relates to a device for the carrying out of chemical or biological reactions with a reaction vessel receiving element for receiving a microtiter plate with several reaction vessels, wherein the reaction vessel receiving element has several recesses arranged in a regular pattern to receive the respective reaction vessels, a heating device for heating the reaction vessel receiving element, and a cooling device for cooling the reaction vessel receiving element. The invention is characterized by the fact that the reaction vessel receiving element is divided into several segments. The individual segments are thermally decoupled from one another, and each segment is assigned a heating device which may be actuated independently of the others. By means of the segmentation of the reaction vessel receiving element, it is possible for zones to be set and held at different temperatures. Since the reaction vessel receiving element is suitable for receiving standard microtiter plates, the device according to the invention may be integrated in existing process sequences.
1. A device for performing biological reactions in a nucleic acid sample, the device comprising:
a reaction vessel receiving element comprising a plurality of reaction vessel holders, the plurality of reaction vessel holders comprising definable groups of reaction vessel holders;
a plurality of Peltier elements, each Peltier element configured to heat and cool a group of reaction vessel holders;
a gastight jacket containing a fluid changeable between a liquid state and a gaseous state, the gastight jacket being configured to provide temperature equalization among the reaction vessel holders;
a cooling element having a first end facing toward the reaction vessel receiving element and a second end facing away from the reaction vessel receiving element;
a heat conducting foil in contact with the reaction vessel receiving element, the heat conducting foil being disposed between the reaction vessel receiving element and each Peltier element, and further comprising heat conducting foil disposed between each Peltier element and the cooling element; and
a controller configured to cycle the device through a predetermined time-temperature profile via heating and cooling.
2. The device of claim 1 , further comprising a base plate extending from the reaction vessel holders.
3. The device of claim 1 , wherein the gastight jacket comprises a pipe.
4. The device of claim 1 , wherein the fluid in the gastight jacket is evaporated and condensed in the gastight jacket.
5. The device of claim 4 , wherein the fluid is evaporated in the gastight jacket at a highest temperature of the reaction vessel receiving element and condensed in the gastight jacket at a lowest temperature of the reaction vessel receiving element.
6. The device of claim 1 , wherein the reaction vessel holders comprise an open end and a closed end, and wherein the gastight jacket is disposed so as to dissipate heat proximate the closed ends of the reaction vessel holders.
7. The device of claim 1 , wherein the gastight jacket comprises a plurality of gastight jackets, each gastight jacket being associated with a differing group of reaction vessel holders.
8. The device of claim 1 , wherein the device is configured to perform a polymerase chain reaction of a nucleic acid sample.
9. The device of claim 1 , wherein each Peltier element is disposed between the reaction vessel holders and the cooling element.