Compositions of prokaryotic phenylalanine ammonia-lyase and methods of using compositions thereof
View Patent ↗The present invention is directed to phenylalanine ammonia lyase (PAL) produced by prokaryotes, wherein such prokaryotic PAL wherein the PAL variant has a greater phenylalanine-converting activity as compared to a wild-type PAL. The invention thus provides compositions of bacterial PAL and biologically active fragments, mutants, variants and analogs thereof, as well as methods for the production, purification, and use of such compositions for therapeutic and industrial purposes.
1. A wild-type Anabaena variabilis phenylalanine ammonia lyase comprising a polyethylene glycol.
2. The phenylalanine ammonia lyase of claim 1 having a phenylalanine ammonia lyase:polyethylene glycol ratio of 1:3.
3. A pharmaceutical composition comprising the phenylalanine ammonia lyase of claim 1 and a pharmaceutically acceptable carrier.
4. A wild-type Nostoc punctiforme phenylalanine ammonia lyase comprising a polyethylene glycol.
5. The phenylalanine ammonia lyase of claim 4 having a phenylalanine ammonia lyase:polyethylene glycol ratio of 1:3.
6. A pharmaceutical composition comprising the phenylalanine ammonia lyase of claim 4 and a pharmaceutically acceptable carrier.