IP Library › Patent Application 11458888
Patent Application
App. No. 11/458,888

COMPOSITIONS AND METHODS FOR ANALYSIS OF TARGET ANALYTES

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Quick Facts
Patent No.
US None
App. No.
11/458,888
Abstract

Compositions and methods are provided for analyzing a sample for the presence or absence of one or more target analytes.

Claims (8)

1 . A method of detecting a target analyte, comprising: a) inhibiting antibody—target analyte binding with a microparticle comprising a competitive inhibitor of said target analyte, and b) measuring the antibody bound to said competitive inhibitor as said microparticle is drawn through a microcapillary cytometer that is optically linked to a fluorescence system, whereby said target analyte is detected.

2 . The method according to claim 1 , wherein said antibody comprises a fluorescent moiety.

3 . The method according to claim 1 , further comprising labeling said antibody bound to said competitive inhibitor with a fluorescent moiety.

4 . The method according to claim 3 , wherein said antibody is labeled by binding said antibody to a second antibody comprising said fluorescent moiety.

5 . A method of detecting a target analyte, comprising: a) inhibiting antibody—target analyte binding with a microparticle comprising a competitive inhibitor of said target analyte, and b) measuring the antibody bound to said competitive inhibitor as said microparticle is drawn through a flow cytometer that is optically linked to a fluorescence system, whereby said target analyte is detected.

6 . The method according to claim 5 , wherein said antibody comprises a fluorescent moiety.

7 . The method according to claim 5 , further comprising labeling said antibody bound to said competitive inhibitor with a fluorescent moiety.

8 . The method according to claim 7 , wherein said antibody is labeled by binding said antibody to a second antibody comprising said fluorescent moiety.