METHOD, APPARATUS, AND SYSTEM FOR EXTENDING DEPTH OF FIELD (DOF) IN A SHORT-WAVELENGTH MICROSCOPE USING WAVEFRONT ENCODING
A lens assembly for enhancing the depth of field of a short-wavelength microscopic system is disclosed. The lens assembly includes an objective zone plate lens, an encoding lens, an imaging detector and a decoding component connected to the imaging detector. The objective zone plate lens is oriented to receive short-wavelength radiation that has passed through a sample in a microscopic system. The encoding lens is oriented to receive the short-wavelength radiation that has passed through the objective zone plate lens and encode the radiation to output an encoded short-wavelength radiation. The imaging detector is oriented to receive the encoded short-wavelength radiation and convert it to a digital signal which is subsequently decoded by the decoding component to decode the encoding applied to the short-wavelength radiation.
1 . A lens assembly for enhancing the depth of field of a short-wavelength microscopic system, comprising:
an objective zone plate lens oriented to receive short-wavelength radiation that has passed through a sample in a microscopic system;
an encoding lens oriented to receive the short-wavelength radiation that has passed through the objective zone plate lens, the encoding lens configured to encode the short-wavelength radiation to output an encoded short-wavelength radiation;
an imaging detector oriented to receive the encoded short-wavelength radiation, the imaging detector configured to convert the encoded short-wavelength radiation to a digital signal; and
a decoding component connected to the imaging plate and configured to process the digital signal to decode the encoding applied to the short-wavelength radiation.
2 . The lens assembly for enhancing the depth of field of a short-wavelength microscopic system, as recited in claim 1 , wherein the encoding applied results in a phase shift of the short-wavelength radiation.
3 . The lens assembly for enhancing the depth of field of a short-wavelength microscopic system, as recited in claim 2 , wherein the decoding component applies an algorithm to the digital signal to reverse the phase shift applied to the short-wavelength radiation.
4 . The lens assembly for enhancing the depth of field of a short-wavelength microscopic system, as recited in claim 1 , wherein the imaging detector is one of a charged-coupled device (CCD) array or an active pixel sensor array.
5 . The lens assembly for enhancing the depth of field of a short-wavelength microscopic system, as recited in claim 1 , wherein the encoding lens is a cubic phase plate lens.
6 . The lens assembly for enhancing the depth of field of a short-wavelength microscopic system, as recited in claim 5 , wherein the cubic phase plate lens is fabricated from a material that is selected from a group consisting of a polymer-based substrate, a zinc sulfide substrate, and a nickel coated silicon nitride substrate.
7 . A short-wavelength microscopic device, comprising:
a laser device configured to emit laser pulses;
a target positioned to receive the laser pulses and configured to convert the laser pulses into short-wavelength radiation;
a condenser zone plate operable to receive short-wavelength radiation and form a diffraction pattern having a focal spot;
a sample stage onto which a specimen sample can be mounted, wherein the sample stage is operable to be positioned at the focal spot;
an objective zone plate operable to receive the short-wavelength radiation that has passed through the specimen sample;
an encoding lens oriented to receive the short-wavelength radiation that has passed through the objective zone plate, the encoding lens configured to encode the short-wavelength radiation to output an encoded short-wavelength radiation;
an imaging detector oriented to receive the encoded short-wavelength radiation, the imaging detector configured to convert the encoded short-wavelength radiation to a digital signal; and
a decoding component connected to the imaging plate and configured to process the digital signal to decode the encoding applied to the short-wavelength radiation.
8 . The short-wavelength microscopic device, as recited in claim 7 , wherein the laser pulses have a wavelength of between about 2.3 nanometers (nm) and about 4.4 nm.
9 . The short-wavelength microscopic device, as recited in claim 7 , wherein the encoding applied results in a phase shift of the short-wavelength radiation.
10 . The short-wavelength microscopic device, as recited in claim 8 , wherein the decoding component applies an algorithm to the digital signal to reverse the phase shift applied to the short-wavelength radiation.
11 . The short-wavelength microscopic device, as recited in claim 7 , wherein the imaging detector is one of a charged-coupled device (CCD) array or an active pixel sensor array.
12 . The short-wavelength microscopic device, as recited in claim 7 , wherein the encoding lens is a cubic phase plate lens.
13 . The short-wavelength microscopic device, as recited in claim 12 , wherein the cubic phase plate lens is fabricated from a material that is selected from a group consisting of a polymer-based substrate, a zinc sulfide substrate, and a nickel coated silicon nitride substrate.
14 . The short-wavelength microscopic device, as recited in claim 7 , wherein the material used to fabricate the target is one of copper or tin.
15 . A method for increasing the depth field in a short-wavelength microscopic device, comprising:
providing a short-wavelength microscope device, the microscope device including,
a condenser zone plate operable to receive short-wavelength radiation and form a diffraction pattern having a first order focal spot,
a sample stage onto which a specimen sample can be mounted, wherein the sample stage is operable to be positioned at the first order focal spot, and
an objective zone plate lens operable to receive short-wavelength radiation that has passed through the specimen sample and focus the short-wavelength radiation onto an encoding element, the encoding element configured to encode the short-wavelength radiation to output an encoded short-wavelength radiation;
positioning an imaging detector to receive the encoded short-wavelength radiation;
transforming the encoded short-wavelength radiation into a digital signal;
sending the digital signal to a decoding component; and
reversing the encoding applied to the short-wavelength radiation using the decoding component.
16 . The method for increasing the depth field in a short-wavelength microscopic device, as recited in claim 15 , wherein the encoding applied results in a phase shift of the short-wavelength radiation.
17 . The method for increasing the depth field in a short-wavelength microscopic device, as recited in claim 16 , wherein the decoding component applies an algorithm to the digital signal to reverse the phase shift applied to the short-wavelength radiation.
18 . The method for increasing the depth field in a short-wavelength microscopic device, as recited in claim 15 , wherein the imaging detector is one of a charged-coupled device (CCD) array or an active pixel sensor array.
19 . The method for increasing the depth field in a short-wavelength microscopic device, as recited in claim 15 , wherein the encoding element is a cubic phase plate lens.
20 . The method for increasing the depth field in a short-wavelength microscopic device, as recited in claim 19 , wherein the cubic phase plate lens is fabricated from a material that is selected from a group consisting of a polymer-based substrate, a zinc sulfide substrate, and a nickel coated silicon nitride substrate.