Preventing transfusion related complications in a recipient of a blood transfusion
View Patent ↗This invention is directed toward a process for reducing transfusion related complications in a recipient of an allogeneic blood transfusion by adding to the blood to be transfused a photosensitizer comprising riboflavin, irradiating the blood and riboflavin with light, transfusing the irradiated blood into a recipient, and reducing a transfusion related complication by the recipient to cells in the donor blood. The invention is also directed towards a process for preventing rejection of a donor organ by a recipient comprising the steps of transfusing the recipient of the donor organ with treated platelets; and transplanting the donor organ into the recipient.
1. A process for reducing transfusion related complications in a human recipient due to human donor cells that may be present in an allogeneic blood transfusion comprising the steps of:
adding to the blood to be transfused a photosensitizer consisting essentially of riboflavin at a concentration of 50 μM;
irradiating the blood and riboflavin with light at a wavelength between 290-370 nm for around 8 minutes to reduce transfusion related complications in the recipient caused by the donor cells;
wherein the transfusion related complications are alloimmunization, Transfusion Associated Graft vs. Host Disease (TA-GvHD) or microchimerism;
transfusing the irradiated blood into a recipient; and
reducing transfusion related complications in the recipient.
2. The process of claim 1 wherein the blood to be transfused is whole blood.
3. The process of claim 1 wherein the blood to be transfused is platelets.
4. The process of claim 1 wherein the donor cells causing transfusion related complications comprise white blood cells.
5. The process of claim 1 wherein the donor cells causing transfusion related complications comprise platelets.
6. The process of claim 1 wherein the step of reducing transfusion related complications further comprises reducing alloimmunization by reducing activation of the donor cells in the recipient.
7. The process of claim 1 wherein the step of reducing transfusion related complications further comprises reducing alloimmunization by reducing proliferation of the donor cells in the recipient.
8. The process of claim 1 wherein the step of reducing transfusion related complications further comprises reducing alloimmunization by reducing production of cytokines by the donor cells in the recipient.
9. The process of claim 1 wherein the step of reducing transfusion related complications further comprises reducing microchimerism by reducing production of antibodies by the donor cells in the recipient.