Serial section analysis for computer-controlled microscopic imaging
View Patent ↗Automated projection of serial section images in microscopic imaging systems. A computer-controlled microscope that is connected electrically to a controller that has a display device. A batch of serial section slides includes a series of serial section slides, each of which has a microscope slide, a sample, and an interesting area. If one of the images is misregistered, then the images may be transformed.
1. A method, comprising:
scanning a plurality of serial section slides with an automated imaging device to produce an image of each slide, each slide including a sample having at least one area related to a corresponding area of the sample of another one of the plurality of serial section slides;
using a computer to identify the related areas of the samples in each image; and
using the computer to transform automatically a position of at least one of the related areas in at least one of the images based on the position of the related area in another one of the images, wherein said using the computer to transform automatically comprises dividing an image into a number of sub images, and determining a positional offset between different portions of the sub images.
2. A method as in claim 1 , wherein said using a computer comprises identifying one of said serial section slides as an anchor slide, and transforming the images of other slides to have positions that are substantially aligned with the image of said anchor slide.
3. A method as in claim 1 , wherein said transform comprises calculating an axial transform for at least one of said serial slides, and transforming the image of said serial slide along said axis, and displaying the transformed image along with the non-transformed image.
4. A method as in claim 1 , further comprising allowing manual adjustments to at least one image after said using the computer to transform.
5. A method as in claim 1 , wherein said using the computer comprises calculating a number of transformations of the image in advance, and using said transformations at a later time.
6. A method as in claim 2 , wherein said transforming comprises identifying a shape in said anchor slide, and aligning said shape in the images of other slides with substantially the same shape in the image of said anchor slide.
7. A system, comprising:
an automated imaging device for scanning a plurality of serial section slides to produce an image of each slide, each slide including a sample having at least one area related to a corresponding area of the sample of another one of the plurality of serial section slides; and
a computer programmed with an algorithm for identifying the related areas of the samples in each image and automatically transforming a position of at least one of the related areas in at least one of the images based on the position of the related area in another one of the images, wherein said automatically transforming comprises dividing an image into a number of sub images, and determining a positional offset between different portions of the sub images.
8. The system of claim 7 , wherein said algorithm further identifies an anchor slide, and changes the image of at least one slide other than said anchor slide to have the same orientation as the image of said anchor slide.
9. The system of claim 7 , wherein said algorithm further identifies an anchor slide, and changes the image of at least one slide other than said anchor slide to have a specified part in the same position as said specified part in the image of said anchor slide.
10. The system of claim 8 , wherein said algorithm calculates a plurality of coordinate transformations at a first time, based on said anchor slide, and uses at least one of said coordinate transformations at a second time, subsequent to said first time.