Apparatus and method of extracting and optically analyzing an analyte from a fluid-based sample
View Patent ↗An extraction and analysis device includes a microfluidic based collection system that extracts one or more different analytes from a fluid-based sample and an optical analysis system directly coupled to the collection system to perform optical analysis on the one or more collected analytes. The microfluidic based collection system includes microfluidic circuitry for directing a fluid based sample to a purification chip. Analytes collected within the purification chip can be either subsequently removed and analyzed or the analytes can be analyzed directly, while still within the purification chip, using the optical analysis system. The purification chip is preferably comprised of a plurality of pillars, the surface area of each pillar is coated with a specific capture chemistry. The specific capture chemistry is applied by derivitizing the pillars such that a ligand, such as a nucleic acid, an amptimer, or an antibody is attached to each pillar.
1. A method of performing a PCR reaction comprising:
a. providing a PCR apparatus comprising a purification chip including a chamber having a plurality of raised structures, each raised structure is coated with a capture moiety to bind with one or more different nucleic acids, and a thermally controlled device coupled to the plurality of raised structures to thermally control the plurality of raised structures;
b. providing PCR reagents to the chamber within the purification chip; and
c. performing a PCR reaction within the purification chip by alternatively raising and lowering a temperature of the plurality of raised structures, thereby alternatively raising and lowering a temperature of one or more substances either attached to the plurality of raised structures or in-solution surrounding the plurality of raised structures.
2. The method of claim 1 further comprising detecting one or more different amplified nucleic acids resulting from the PCR reaction, wherein the one or more different amplified nucleic acids are detected in the chamber.
3. The method of claim 2 wherein detecting the one or more different amplified nucleic acids includes directing light into the chamber and to capture light in response to the directed light.
4. The method of claim 1 wherein detecting the one or more different amplified nucleic acids includes detecting one or more optical characteristics associated with the one or more different amplified nucleic acids.
5. The method of claim 4 wherein detecting the one or more different amplified nucleic acids includes performing one or more of a fluorescence method, a chi-luminescence method, or a time resolved fluorescence method to detect the one or more optical characteristics.
6. The method of claim 1 wherein raising the temperature breaks the binding between the raised structure and the one or more different nucleic acids bound to the raised structure.
7. The method of claim 2 wherein lowering the temperature enables the one or more different nucleic acids to bind to the raised structures.
8. The method of claim 1 wherein the PCR apparatus further comprises one or more solution containers for storing the PCR reagents.
9. The method of claim 8 wherein the PCR apparatus further comprises microfluidic circuitry coupled to the one or more solution containers and the chamber to transport the PCR reagents.