Micellar systems
Methods are described for modifying nucleic acids to facilitate delivery of the nucleic acids to cells. Compounds which interact with of modify nucleic acids are interacted with the nucleic acids within reverse micelles.
1 . A process for modifying a nucleic acid comprising:
a) forming a reverse micelle containing the nucleic acid;
b) adding a nucleic acid modifying agent to the nucleic acid in the reverse micelle;
c) disrupting the reverse micelle; and,
d) recovering the modified nucleic acid.
2 . The process of claim 1 wherein the nucleic acid contains a reactive group.
3 . The process of claim 2 wherein the reactive group consists of a cysteine.
4 . A process for condensing a nucleic acid comprising:
a) forming a reverse micelle containing the nucleic acid;
b) adding a polycation to the nucleic acid in the reverse micelle to form a condensed nucleic acid-polycation complex;
c) disrupting the reverse micelle; and,
d) recovering the nucleic acid-polycation complex.
5 . The process of claim 4 further comprising adding a modifying agent to the nucleic acid-polycation complex in the reverse micelle.
6 . The process of claim 5 wherein the modifying agent consists of a crosslinker.