Method of combing a nucleic acid
View Patent ↗Elongated molecules are stretched across a substrate by controlled fluid flow.
1. A method of applying a nucleic acid to a surface, comprising:
applying a nucleic acid-containing solution to a first substrate, the first substrate having a defined local physical feature spatially fixed thereon, the defined local physical feature comprising a nucleic acid anchor; and
controllably flowing a liquid deposition fluid over the first substrate by applying a cover surface to the deposition fluid in a manner that promotes stretching of a nucleic acid molecule from the defined local physical feature along the first substrate, wherein controllably flowing further comprises inducing surface charges on at least one of the cover surface and the first substrate.
2. The method of claim 1 , wherein inducing surface charges comprises photoinducing surface charges.
3. The method of claim 1 , wherein the induced surface charges are static.
4. The method of claim 1 , wherein the induced surface charges are dynamic.
5. The method of claim 1 , wherein the induced surface charges are spatially patterned.
6. A method of applying a nucleic acid to a surface, comprising:
applying a nucleic acid-containing solution to a first substrate, the first substrate having a defined local physical feature spatially fixed thereon, the defined local physical feature comprising a nucleic acid anchor; and
controllably flowing a liquid deposition fluid over the first substrate by applying a cover surface to the deposition fluid in a manner that promotes stretching of a nucleic acid molecule from the defined local physical feature along the first substrate, wherein controllably flowing comprises directing fluid flow by controlling hydrophilicity of at least one of the cover surface and the first substrate.
7. A method of applying a nucleic acid to a surface, comprising:
applying a nucleic acid-containing solution to a first substrate, the first substrate having a defined local physical feature spatially fixed thereon, the defined local physical feature comprising a nucleic acid anchor; and
controllably flowing a liquid deposition fluid over the first substrate in a manner that promotes stretching of a nucleic acid molecule from the defined local physical feature along the first substrate, wherein controllably flowing comprises inducing voracity into the deposition fluid.
8. The method of claim 7 , wherein the fluid flow promotes deposition of a nucleic acid molecule in a curve along the substrate.
9. A method of applying a nucleic acid to a surface, comprising:
applying a nucleic acid-containing solution to a first substrate, the first substrate having a defined local physical feature spatially fixed thereon, the defined local physical feature comprising a nucleic acid anchor; and
controllably flowing a liquid deposition fluid over the first substrate in a manner that promotes stretching of a nucleic acid molecule from the defined local physical feature along the first substrate, wherein controllably flowing comprises directing fluid flow by applying a varying surface tension along the first substrate.
10. A method of applying a nucleic acid to a surface, comprising:
applying a nucleic acid-containing solution to a first substrate, the first substrate having a defined local physical feature spatially fixed thereon, the defined local physical feature comprising a nucleic acid anchor; and
controllably flowing a liquid deposition fluid over the first substrate in a manner that promotes stretching of a nucleic acid molecule from the defined local physical feature along the first substrate, wherein controllably flowing comprises directing fluid flow by controlling hydrophilicity of the first substrate.
11. The method of claim 10 , wherein controlling hydrophilicity of the first substrate comprises dynamically changing the hydrophilicity of the first substrate.
12. A method of applying a nucleic acid to a surface, comprising:
applying a nucleic acid-containing solution to a first substrate, the first substrate having a defined local physical feature spatially fixed thereon, the defined local physical feature comprising a nucleic acid anchor; and
controllably flowing a liquid deposition fluid over the first substrate in a manner that promotes stretching of a nucleic acid molecule from the defined local physical feature along the first substrate, wherein controllably flowing comprises varying the temperature of the deposition fluid.