Postpartum cells derived from umbilical cord tissue, and methods of making and using the same
Cells derived from human umbilical cords are disclosed along with methods for their therapeutic use. Isolation techniques, culture methods and detailed characterization of the cells with respect to their cell surface markers, gene expression, and their secretion of trophic factors are described.
1 . An isolated human umbilicus-derived cell capable of self-renewal and expansion in culture and having the potential to differentiate into cells of other phenotypes, wherein the cell does not substantially stimulate naïve CD4 + T cells, and expresses PD-L2, but not HLA-G, CD 178, HLA-DR, HLA-DP, HLA-DQ, CD80, CD86, or B7-H2.
2 . The isolated cell of claim 1 wherein the cell produces vimentin and alpha-smooth muscle actin.
3 . The isolated cell of claim 1 wherein the cell secretes one or more cellular factors.
4 . The isolated cell of claim 3 wherein the factors are MCP-1, IL-6, IL-8, GCP-2, HGF, KGF, FGF, HB-EGF, BDNF, TPO, or TIMP1.
5 . The isolated cell of claim 4 wherein the cell secretes each of MCP-1, IL-6, IL-8, GCP-2, HGF, KGF, FGF, HB-EGF, BDNF, TPO, and TIMP1.
6 . The isolated cell of claim 1 wherein the cell does not secrete one or more of the cellular factors SDF-1alpha, TGF-beta2, ANG2, PDGFbb or VEGF, as detected by ELISA.
7 . The isolated cell of claim 6 , wherein the cell secretes one or more of the factors MCP-1, IL-6, IL-8, GCP-2, HGF, KGF, FGF, HB-EGF, BDNF, TPO, and TIMP1.
8 . The isolated cell of claim 7 wherein the cell secretes each of the factors MCP-1, IL-6, IL-8, GCP-2, HGF, KGF, FGF, HB-EGF, BDNF, TPO, and TIMP1.
9 . The isolated cell of claim 6 wherein the cell does not secrete any of the factors SDF-1alpha, TGF-beta2, ANG2, PDGFbb or VEGF, as detected by ELISA.
10 . The isolated cell of claim 9 , wherein the cell secretes one or more of the factors MCP-1, IL-6, IL-8, GCP-2, HGF, KGF, FGF, HB-EGF, BDNF, TPO, or TIMP1.
11 . The isolated cell of claim 10 wherein the cell secretes each of the factors MCP-1, IL-6, IL-8, GCP-2, HGF, KGF, FGF, HB-EGF, BDNF, TPO, and TIMP1.
12 . The isolated cell of claim 7 wherein the cell does not secrete any of the factors SDF-1alpha, TGF-beta2, ANG2, PDGFbb or VEGF, as detected by ELISA.
13 . An injectable therapeutic cell comprising an isolated human umbilicus-derived cell of claim 1 .
14 . The injectable cell of claim 13 treated to inactivate tissue factor.
15 . The injectable cell of claim 14 wherein the treated is with an anti-tissue factor antibody.