Arrangement for the detection of illumination radiation in a laser scanning microscope
View Patent ↗An arrangement for the detection of the illumination radiation in a laser scanning microscope, wherein a portion of the illumination radiation is coupled out and detected at the main color splitter, wherein light transmitted through the main color splitter is advantageously detected and/or a portion of the illumination radiation is coupled out and detected before coupling into a light-conducting fiber and/or a portion of the illumination radiation is coupled out and detected at a beam splitter arranged downstream of a light-conducting fiber.
1. An arrangement for the detection of the illumination radiation in a laser scanning microscope, comprising:
a laser module having a plurality of laser sources;
an illumination module coupled to the laser module by a plurality of light-conducting fibers, said illumination module having displaceable collimating optics and beam splitters, each beam splitter being associated with a respective optical fiber;
a main color splitter;
a number of monitoring diodes corresponding to the number of beam splitters, each monitoring diode being positioned behind a respective beam splitter in illumination direction,
each beam splitter, other than the main color splitter, reflecting illumination radiation coming from the respective optical fiber to the main color splitter and also permitting pass through illumination radiation coming from the respective optical fiber to the respective monitoring diode;
said beam splitters and monitoring diodes are arranged in such a way that the illumination radiation directed to the monitoring diodes has the same polarization direction as the illumination directed to the sample by the main color splitter;
wherein the illumination module is positioned so that the beam splitters direct the illumination radiation from the collimating optics to the main color splitter and monitoring diodes,
wherein the monitoring diodes detect different wavelengths of the illumination radiation.
2. The arrangement according to claim 1 , wherein light transmitted through an input-coupling splitter is detected.
3. The arrangement according to claim 1 , wherein the illumination module further comprises a plurality monitoring diodes, wherein each one of the plurality of monitoring diodes monitors the wavelength of the illumination radiation received from a respective laser port.