Process for removing protein aggregates and virus from a protein solution
View Patent ↗A process is provided for selectively removing protein aggregates from a protein solution in a normal flow (NFF) filtration process. Preferably, it relates to a process for selectively removing protein aggregates from a protein solution in a normal flow (NFF) filtration process and virus particles from a protein solution in a two-step filtration process. In a first step, a protein solution is filtered through one or more layers of adsorptive depth filters, charged or surface modified microporous membranes or a small bed of chromatography media in a normal flow filtration mode of operation, to produce a protein aggregate free stream. The aggregate free protein stream can then be filtered through one or more ultrafiltration membranes to retain virus particles at a retention level of at least 3 LRV and to allow passage therethrough of an aggregate free and virus free protein solution.
1. A process for viral filtration of a protein containing stream comprising:
(a) providing a filtration system comprising: (i) a pressurized reservoir comprising a solution which comprises one or more protein aggregates and viruses; (ii) a first conduit connecting the reservoir to a first filtration step comprising a device selected from the group consisting of one or more layers of adsorptive depth filters and one or more layers of charged or surface modified microporous membranes; (iii) a second conduit from the first filtration step to a second filtration step comprising one or more ultrafiltration membranes having a molecular weight cut off of between about 200 kD and about 1000 kD to retain virus particles in said one or more ultrafiltration membranes at a level of at least 3 LRV; and (d) an outlet conduit connected to the second filtration step;
(b) pumping the solution from the pressurized reservoir through the first conduit to the first filtration step;
(c) running the first filtration step in a normal flow filtration mode with the one or more protein aggregates and viruses being retained by the first filtration step;
(d) discharging the solution from the first filtration step to the second conduit;
(e) passing the solution from the second conduit through the second filtration step; and
(f) discharging the solution from the second filtration step to the outlet conduit.
2. The process of claim 1 wherein the process is run in a constant pressure mode of operation.
3. The process of claim 1 further comprising the step of flushing retained protein from said one or more ultrafiltration membranes.
4. The process of claim 1 wherein the filtration is through one or more layers of adsorptive depth filters.
5. The process of claim 1 wherein the filtration is through one or more layers of one or more layers of charged or surface modified microporous membranes.
6. The process of claim 1 wherein the filtration is through one or more layers of adsorptive depth filters made of a material selected from the group consisting of cellulosic fibers, synthetic fibers and blends thereof.
7. A process for the viral filtration of a protein containing stream comprising:
(a) providing a filtration system comprising a non-pressurized reservoir comprising a solution which comprises one or more protein aggregates and viruses; (ii) a first conduit connecting the reservoir to a pump, wherein the pump is connected to a second conduit which connects the pump to a first filtration step comprising a device selected from the group consisting of one or more layers of adsorptive depth filters and one or more layers of charged or surface modified microporous membranes; (iii) a third conduit from the first filtration step to a second filtration step comprising one or more ultrafiltration membranes having a molecular weight cut off of between about 200 kD and about 1000 kD to retain virus particles in said one or more ultrafiltration membranes at a level of at least 3 LRV; and (iv) an outlet conduit from the second filtration step;
(b) pumping the solution from the reservoir through the first conduit to the pump and from the pump through the second conduit to the first filtration step;
(c) running the first filtration step in a normal flow filtration mode with the one or more protein aggregates and viruses being retained by the first filtration step;
(d) discharging the solution from the first filtration step to the third conduit;
(e) passing the solution from the third conduit through the second filtration step in a normal flow filtration mode; and
(f) discharging the solution from the second filtration step to the outlet conduit.
8. The process of claim 7 wherein the process is run in a constant flow mode of operation.
9. The process of claim 7 further comprising the step of flushing retained protein from said one or more ultrafiltration membranes.
10. The process of claim 7 wherein filtration with said one or more ultrafiltration membranes is effected by tangential flow filtration.
11. The process of claim 7 wherein filtration with said one or more ultrafiltration membranes is effected in a normal flow filtration mode of operation.
12. The process of claim 7 wherein the first filtration step is through one or more layers of adsorptive depth filters.
13. The process of claim 7 wherein the first filtration step is through one or more layers of charged or surface modified microporous membranes.
14. The process of claim 7 wherein the first filtration step is through one or more layers of adsorptive depth filters made of a material selected from the group consisting of cellulosic fibers, synthetic fibers and blends thereof.