RNAi modulation of the Rho-A gene in research models
The invention relates to compositions and methods for modulating the expression of the RhoA gene, and more particularly to the downregulation of RhoA by chemically modified oligonucleotides.
1 . A method of stimulating neuronal cell outgrowth in a neural cell culture comprising contacting the neural cell culture with an iRNA agent, wherein the iRNA agent comprises (i) a sense strand, wherein the sense strand comprises at least 15 contiguous nucleotides that differ by no more than 1, 2, or 3 nucleotides from the sense strand sequences of any one agent selected from the group consisting of agents number 5850 to 6177, and (ii) an antisense strand, wherein the antisense strand comprises at least 15 contiguous nucleotides that differ by no more than 1, 2, or 3 nucleotides from the antisense sequences of any one agent selected from the group consisting of agents number 5850 to 6177.
2 . A method of performing an animal study comprising:
(a) providing an animal having a neural disfunction;
(b) administering an iRNA agent wherein the iRNA agent comprises (i) a sense strand, wherein the sense strand comprises at least 15 contiguous nucleotides that differ by no more than 1, 2, or 3 nucleotides from the sense strand sequences of any one agent selected from the group consisting of agents number 5850 to 6177, and (ii) an antisense strand, wherein the antisense strand comprises at least 15 contiguous nucleotides that differ by no more than 1, 2, or 3 nucleotides from the antisense sequences of any one agent selected from the group consisting of agents number 5850 to 6177; and
(c) monitoring the animal for an improvement in neural function.