Antibody profiles characteristic of tuberculosis state
View Patent ↗Serum antibody assays capable of distinguishing cases of inactive TB from cases of active TB include a combination of at least three M. tuberculosis protein antigens, at least one for which a positive response is consistent with inactive TB and at least one for which a negative response is consistent with inactive TB. Preferred assays further distinguish other TB classes. Also, antigen kits for performing such assays.
1. An assay for tuberculosis that is performed on a serum sample from a human subject, wherein the assay is capable of determining the likelihood that the subject has active tuberculosis or inactive tuberculosis, the assay comprising exposing antibodies in the sample to at least three different M. tuberculosis protein antigens separately immobilized on different locations on a surface, wherein the at least three different M. tuberculosis protein antigens include at least one antigen of a first type selected from the group consisting of 16 kDa antigen (product of gene rv 2031c) and ESAT-6 antigen (product of gene rv 3875), at least one antigen of a second type selected from the group consisting of Rv2626c antigen, AlaDH antigen (product of gene rv 2780) and 38 kDa antigen (product of gene rv 0934), and at least one antigen of a third type selected from the group consisting of 16 kDa antigen (product of gene rv 2031c), ESAT-6 antigen (product of gene rv 3875), Rv2626c antigen, AlaDH antigen (product of gene rv 2780) and 38 kDa antigen (product of gene rv 0934), and detecting serum antibodies to said antigens.
2. The assay according to claim 1 that includes at least 16 kDa antigen and ESAT-6 antigen and at least two antigens of the second type.
3. The assay of claim 1 that includes at least 16 kDa antigen and ESAT-6 antigen, further comprising FdxA antigen (product of gene rv 2007c).
4. A kit of reagents for performing a tuberculosis assay that is capable of determining the likelihood that a subject has active tuberculosis or inactive tuberculosis, said kit comprising at least three different M. tuberculosis protein antigens separately immobilized on different locations on a surface, wherein the at least three different M. tuberculosis protein antigens include at least one antigen of a first type selected from the group consisting of 16 kDa antigen (product of gene rv 2031c) and ESAT-6 antigen (product of gene rv 3875), at least one antigen of a second type selected from the group consisting of Rv2626c antigen, AlaDH antigen (product of gene rv 2780) and 38 kDa antigen (product of gene rv 0934), and at least one antigen of a third type selected from the group consisting of Rv2626c antigen, AlaDH antigen (product of gene rv 2780) and FdxA antigen (product of gene rv 2007c), and reagents for detecting binding of antibodies to said antigens.
5. The kit according to claim 4 that includes at least 16 kDa antigen and ESAT-6 antigen and at least two antigens of the second type.
6. The kit according to claim 4 that includes at least 16 kDa antigen, ESAT-6 antigen, and FdxA antigen (product of gene rv 2007c).