RNA interference mediated inhibition of gene expression using chemically modified short interfering nucleic acid (siNA)
View Patent ↗The present invention concerns methods and reagents useful in modulating gene expression in a variety of applications, including use in therapeutic, diagnostic, target validation, and genomic discovery applications. Specifically, the invention relates to synthetic chemically modified small nucleic acid molecules, such as short interfering nucleic acid (siNA), short interfering RNA (siRNA), double-stranded RNA (dsRNA), micro-RNA (miRNA), and short hairpin RNA (shRNA) molecules capable of mediating RNA interference (RNAi) against target nucleic acid sequences. The small nucleic acid molecules are useful in the treatment of any disease or condition that responds to modulation of gene expression or activity in a cell, tissue, or organism.
1. A chemically modified double stranded nucleic acid molecule that mediates RNA interference (RNAi) against a target RNA sequence, wherein:
a) the nucleic acid molecule comprises a sense strand and a separate antisense strand;
b) each strand of the nucleic acid molecule is independently 18 to 24 nucleotides in length;
c) the antisense strand is complementary to the target RNA sequence; and
d) a plurality of pyrimidine nucleotides in both the sense strand and the antisense strand are 2′-deoxy-2′-fluoro pyrimidine nucleotides, a plurality of purine nucleotides in the sense strand are 2′-deoxy purine nucleotides, and a plurality of purine nucleotides in the antisense strand are 2′-O-methyl purine nucleotides.
2. The nucleic acid molecule of claim 1 , wherein one or more of the chemically modified nucleotides are phosphorothioate linked nucleotides.
3. The nucleic acid molecule of claim 1 , further comprising inverted deoxy abasic modifications at the 3′-end, the 5′-end, or both the 3′- and 5′-ends of the sense strand.
4. The nucleic acid molecule of claim 1 , wherein the sense strand comprises a 3′-overhang of 1-4 nucleotides.
5. The nucleic acid molecule of claim 1 , wherein the antisense strand comprises an inverted deoxy abasic modification at the 3′-end of the antisense strand.
6. The nucleic acid molecule of claim 1 , wherein the antisense strand comprises a 3′-overhang of 1-4 nucleotides.
7. A chemically modified double stranded nucleic acid molecule that mediates RNA interference (RNAi) against a target RNA sequence, wherein:
a) the nucleic acid molecule comprises a sense strand and a separate antisense strand;
b) each strand of the nucleic acid molecule is independently 18 to 24 nucleotides in length;
c) the antisense strand is complementary to the target RNA sequence; and
d) a plurality of pyrimidine nucleotides in both the sense strand and the antisense strand are 2′-deoxy-2′-fluoro pyrimidine nucleotides, a plurality of purine nucleotides in the sense strand are ribonucleotides, and a plurality of purine nucleotides in the antisense strand are 2′-O-methyl purine nucleotides.
8. The nucleic acid molecule of claim 7 , wherein one or more of the chemically modified nucleotides are phosphorothioate linked nucleotides.
9. The nucleic acid molecule of claim 7 , further comprising inverted deoxy abasic modifications at the 3′-end, the 5′-end, or both the 3′- and 5′-ends of the sense strand.
10. The nucleic acid molecule of claim 7 , wherein the sense strand comprises a 3′-overhang of 1-4 nucleotides.
11. The nucleic acid molecule of claim 7 , wherein the antisense strand comprises an inverted deoxy abasic modification at the 3′-end of the antisense strand.
12. The nucleic acid molecule of claim 7 , wherein the antisense strand comprises a 3′-overhang of 1-4 nucleotides.
13. A composition comprising the nucleic acid molecule of claim 1 and a pharmaceutically acceptable carrier or diluent.
14. A composition comprising the nucleic acid molecule of claim 7 and a pharmaceutically acceptable carrier or diluent.