Method of combing a nucleic acid
View Patent ↗Elongated molecules are stretched across a substrate by controlled fluid flow.
1. A method of forming a nanowire, comprising:
applying a nucleic acid-containing solution to a first substrate, the first substrate having a defined structural feature thereon;
controllably flowing the nucleic acid-containing solution over the first substrate in a manner that promotes stretching of a nucleic acid molecule from the defined structural feature along the first substrate; and
applying a conductive material to the stretched nucleic acid molecule to form a nanowire; and
controlling a surface charge of the first substrate, wherein the surface charge acts to attract the nucleic acid molecule to the first substrate.
2. A method of forming a nanowire, comprising:
applying a nucleic acid-containing solution to a first substrate, the first substrate having a defined structural feature thereon;
controllably flowing the nucleic acid-containing solution over the first substrate in a manner that promotes stretching of a nucleic acid molecule from the defined structural feature along the first substrate; and
applying a conductive material to the stretched nucleic acid molecule to form a nanowire; and
applying a cover surface to the nucleic acid-containing solution, wherein controllably flowing comprises moving the cover surface relative to the first substrate to induce fluid flow in the nucleic acid-containing solution.
3. A method of forming a nanowire, comprising:
applying a nucleic acid-containing solution to a first substrate, the first substrate having a defined structural feature thereon;
controllably flowing the nucleic acid-containing solution over the first substrate in a manner that promotes stretching of a nucleic acid molecule from the defined structural feature along the first substrate;
applying a conductive material to the stretched nucleic acid molecule to form a nanowire; and
applying a cover surface to the nucleic acid-containing solution, wherein controllably flowing comprises moving the cover surface relative to the first substrate to induce fluid flow in the nucleic acid-containing solution.
4. The method of claim 3 , wherein moving the cover surface relative to the first substrate comprises peeling the cover surface away from the first substrate.
5. The method of claim 3 , wherein moving the cover surface relative to the first substrate comprises sliding the cover surface along the first substrate.
6. The method of claim 3 , wherein at least one of the cover surface and the first substrate comprises surface features that spatially direct the fluid flow.
7. The method of claim 3 , wherein controllably flowing further comprises inducing surface charges on at least one of the cover surface and the first substrate.
8. The method of claim 7 , wherein inducing surface charges comprises photo inducing surface charges.
9. The method of claim 3 , wherein controllably flowing further comprises applying a varying surface tension along at least one of the cover surface and the first substrate.
10. A method of forming a nanowire, comprising:
applying a nucleic acid-containing solution to a first substrate, the first substrate having a defined structural feature thereon;
controllably flowing the nucleic acid-containing solution over the first substrate in a manner that promotes stretching of a nucleic acid molecule from the defined structural feature along the first substrate; and
applying a conductive material to the stretched nucleic acid molecule to form a nanowire, wherein controllably flowing comprises controlling a surface charge of the first substrate, wherein the surface charge is dynamic.
11. A method of forming a nanowire, comprising: applying a nucleic acid-containing solution to a first substrate, the first substrate having a defined structural feature thereon;
controllably flowing the nucleic acid-containing solution over the first substrate in a manner that promotes stretching of a nucleic acid molecule from the defined structural feature along the first substrate; and
applying a conductive material to the stretched nucleic acid molecule to form a nanowire, wherein controllably flowing comprises controlling a surface charge of the first substrate, wherein the surface charge is static.
12. A method of forming a nanowire, comprising:
applying a nucleic acid-containing solution to a first substrate, the first substrate having a defined structural feature thereon;
controllably flowing the nucleic acid-containing solution over the first substrate in a manner that promotes stretching of a nucleic acid molecule from the defined structural feature along the first substrate; and
applying a conductive material to the stretched nucleic acid molecule to form a nanowire, wherein controllably flowing comprises directing fluid flow by applying an electromagnetic field to the nucleic acid-containing solution.
13. The method of claim 12 , wherein the electromagnetic field is a pure electric field.
14. The method of claim 12 , wherein the electromagnetic field is a pure magnetic field.
15. The method of claim 12 , wherein the electromagnetic field is a plasmon.
16. A method of forming a nanowire, comprising:
applying a nucleic acid-containing solution to a first substrate, the first substrate having a defined structural feature thereon;
controllably flowing the nucleic acid-containing solution over the first substrate in a manner that promotes stretching of a nucleic acid molecule from the defined structural feature along the first substrate; and
applying a conductive material to the stretched nucleic acid molecule to form a nanowire, wherein controllably flowing comprises directing fluid flow by controlling hydrophilicity of the first substrate.
17. A method of forming a nanowire, comprising:
applying a nucleic acid-containing solution to a first substrate, the first substrate having a defined structural feature thereon;
controllably flowing the nucleic acid-containing solution over the first substrate in a manner that promotes stretching of a nucleic acid molecule from the defined structural feature along the first substrate; and
applying a conductive material to the stretched nucleic acid molecule to form a nanowire, wherein controllably flowing comprises varying the temperature of the nucleic acid-containing solution.
18. A method of forming a nanowire, comprising:
applying a nucleic acid-containing solution to a first substrate, the first substrate having a defined structural feature thereon;
controllably flowing the nucleic acid-containing solution over the first substrate in a manner that promotes stretching of a nucleic acid molecule from the defined structural feature along the first substrate; and
applying a conductive material to the stretched nucleic acid molecule to form a nanowire, wherein the nucleic acid-containing solution comprises a mechanical anchor structure having a nucleic acid molecule anchored thereto, and where the defined structural feature acts to spatially fix the mechanical anchor.
19. A method of forming a nanowire, comprising:
applying a nucleic acid-containing solution to a first substrate, the first substrate having a defined structural feature thereon;
controllably flowing the nucleic acid-containing solution over the first substrate in a manner that promotes stretching of a nucleic acid molecule from the defined structural feature along the first substrate; and
applying a conductive material to the stretched nucleic acid molecule to form a nanowire, wherein the defined structural feature comprises an anchored nucleic acid strand, and wherein nucleic acid in the nucleic acid-containing solution hybridizes to the anchored nucleic acid strand.
20. The method of claim 19 , further comprising dehybridizing the nucleic acid in the nucleic acid-containing solution and the anchored nucleic acid strand.