Hybridoma cell lines and monoclonal antibodies recognizing Prox1
View Patent ↗Monoclonal antibodies to Prox1 of vertebrates (pfam05044.5; NM_002763.3) and two continuous cell lines for their production are disclosed. These antibodies are particularly useful in immunoassays to detect the presence of Prox1 protein in vertebrate tissues and cells.
1. A hybridoma cell line selected from the group consisting of 5G10 (ATCC patent deposit number PTA-6828) and 4G10 (ATCC patent deposit number PTA-6854).
2. The hybridoma cell line of claim 1 , wherein said cell line is 5G10 (ATCC patent deposit number PTA-6828).
3. The hybridoma cell line of claim 1 , wherein said cell line is 4G10 (ATCC patent deposit number PTA-6854).
4. A monoclonal antibody produced by the hybridoma cell line of claim 1 .
5. A monoclonal antibody produced by the hybridoma cell line of claim 2 .
6. A monoclonal antibody produced by the hybridoma cell line of claim 3 .
7. A conjugate comprising an antibody of claim 4 conjugated directly or indirectly to a detectable label.
8. A conjugate comprising an antibody of claim 5 conjugated directly or indirectly to a detectable label.
9. A conjugate comprising an antibody of claim 6 conjugated directly or indirectly to a detectable label.
10. A method of producing an antibody comprising the step of culturing cells of the hybridoma cell line of claim 1 under conditions that permit production of the antibody.
11. A kit comprising: (i) the monoclonal antibody of claim 4 ; (ii) a washing solution; and (iii) a detectable label.
12. The kit of claim 11 further comprising a support to which the monoclonal antibody is attached.
13. The kit of claim 11 , wherein the detectable label is selected from the group consisting of an enzyme, a radiolabel, a color label, a fluorescent label, a chemiluminescent label, a bioluminescent label, and a particulate label.