Novel bacterial insecticidal proteins and equivalents thereof were isolated. These proteins and the DNA sequences encoding them are useful to make insecticidal compositions or transgenic plants to protect plants from damage by insects, particularly coleopteran insects.
1. An isolated DNA encoding a protein comprising the amino acid sequence of SEQ ID NO:4 from amino acid residue 51 to amino acid residue 449 or 457; wherein said protein is insecticidal to Diabrotica virgifera larvae when ingested by said insect in combination with the protein fragment of SEQ ID NO:2 from amino acid residue 38 to amino acid residue 871.
2. An isolated DNA encoding a protein comprising an amino acid sequence with at least 97% sequence identity to the amino acid sequence of SEQ ID NO:4 or encoding a protein comprising the amino acid sequence of SEQ ID NO: 8; wherein said protein is insecticidal to Diabrotica virgifera larvae when ingested by said insect in combination with the protein fragment of SEQ ID NO:2 from amino acid residue 38 to amino acid residue 871.
3. The DNA of claim 1 or 2 , comprising an artificial DNA sequence having a different codon usage compared to the naturally occurring DNA sequence of SEQ ID NO:3.
4. A chimeric gene comprising the DNA of claim 3 operably linked to a plant-expressible promoter.
5. The chimeric gene of claim 4 , wherein said promoter region is preferentially active in root tissue.
6. The chimeric gene of claim 4 wherein said chimeric gene further comprises a signal peptide for secretion from the cell or for targeting to a cellular organelle.
7. The chimeric gene of claim 6 wherein said signal peptide is a chloroplast transit peptide.
8. A chimeric gene comprising the DNA of claim 1 or 2 operably linked to a plant-expressible promoter.
9. The chimeric gene of claim 8 , wherein said promoter region is preferentially active in root tissue.
10. The chimeric gene of claim 8 wherein said chimeric gene further comprises a signal peptide for secretion from the cell or for targeting to a cellular organelle.
11. The chimeric gene of claim 10 wherein said signal peptide is a chloroplast transit peptide.