IP Library › Patent Application 11508006
Patent Application
App. No. 11/508,006

Isolated CEL II endonuclease

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Quick Facts
Patent No.
US None
App. No.
11/508,006
Abstract

The present invention relates to the isolation and characterization of CEL I and CEL II endonuclease proteins. Methods and kits for identifying mismatches in double-stranded DNA are also provided.

Claims (2)

1 . A method for detecting the presence of mismatches in double-stranded DNA comprising contacting a sample containing double-stranded DNA with an isolated CEL II endonuclease, separating the product of the CEL II endonuclease digestion, and detecting said product wherein an increase in the number of DNA fragments generated in the presence of the CEL II endonuclease is indicative of a mismatch in said DNA.

2 . A kit for detecting the presence of mismatches in double-stranded DNA comprising an isolated CEL II endonuclease enzyme.