Methods and compositions for the expression of a polynucleotide of interest
Methods and compositions for expressing a polynucleotide of interest are provided. Compositions comprise an enhancer domain set forth in SEQ ID NO: 1, 10, 15, or 16 and active variants and fragments thereof. Further provided are DNA constructs comprising at least one transcriptional enhancer sequence comprising the nucleotide sequence set forth in SEQ ID NO:1, 10, 15, or 16 or an active variant or fragment thereof, operably linked to a heterologous promoter. Such chimeric transcription regulatory regions can be operably linked any polynucleotide of interest. Further provided are cells, plants, plant parts, and germplasm comprising the DNA construct. Methods of using the chimeric transcriptional regulatory region are also provided. In specific embodiments, methods of expressing a polynucleotide of interest, including for example, sequences that confer tolerance to herbicides, and methods to select a cell having the DNA construct are provided.
1 . A method of expressing a polynucleotide of interest comprising
introducing into a cell at least one DNA construct comprising a chimeric transcriptional regulatory region comprising at least one enhancer domain operably linked to a heterologous promoter, said chimeric transcriptional regulatory region operably linked to the polynucleotide of interest, wherein said enhancer domain is selected from the group consisting of:
(a) the nucleotide sequence comprising SEQ ID NO: 1, 17;
(b) the nucleotide sequence comprising at least 95% sequence identity to SEQ ID NO: 1 or 17, wherein said polynucleotide has transcriptional regulatory activity; and,
(c) the nucleotide sequence comprising at least 100 consecutive nucleotides of SEQ ID NO:1 or 17.
2 . The method of claim 1 , wherein said enhancer domain does not comprise the sequence set forth in SEQ ID NO:5.
3 . The method of claim 1 , wherein said chimeric transcriptional regulatory region comprises at least two copies or at least three copies of said enhancer.
4 . The method of claim 3 , wherein
a) said copies of said enhancer are immediately adjacent to one another; or,
b) at least one of said enhancers is in orientated in the forward or reverse orientation with respect to said promoter.
5 . The method of claim 1 , wherein said polynucleotide of interest encodes a polypeptide.
6 . The method of claim 1 , wherein said polynucleotide of interest comprises a selectable marker.
7 . The method of claim 6 , wherein said selectable marker comprises a polynucleotide that confers tolerance to a herbicide.
8 . The method of any one of claim 1 , wherein said cell is a plant cell.
9 . The method of claim 7 , wherein said method further comprise culturing said cell in a media comprising an effective concentration of the herbicide.
10 . The method of claim 8 , wherein said plant cell is from a dicot.
11 . The method of claim 10 , wherein said dicot is soybean, wheat, canola, sunflower, cotton, or alfalfa.
12 . The method of claim 8 , wherein said plant cell is from a monocot.
13 . The method of claim 12 , wherein said monocot is maize, wheat, rice, barley, sorghum, or rye.
14 . A method to select a cell having a polynucleotide of interest comprising
a) providing a population of cells;
b) introducing into at least one cell from said population a DNA construct comprising the polynucleotide of interest and further comprises a chimeric transcriptional regulatory region comprising at least one enhancer domain operably linked to a first heterologous promoter, said chimeric transcriptional regulatory region operably linked to a selectable marker, wherein said enhancer domain is selected from the group consisting of:
i) the nucleotide sequence comprising SEQ ID NO: 1 or 17;
ii) the nucleotide sequence comprising at least 95% sequence identity to SEQ ID NO: 1 or 17, wherein said polynucleotide has regulating transcriptional activity; and,
iii) the nucleotide sequence comprising at least 100 consecutive nucleotides of SEQ ID NO:1 or 17;
c) contacting said population of cells with an effective concentration of an appropriate selection agent; and,
d) selecting the plant expressing said selectable marker, and thereby identifying plants having the polynucleotide of interest.
15 . The method of claim 14 , wherein said enhancer domain does not comprise the sequence set forth in SEQ ID NO:5.
16 . The method of claim 14 , wherein said chimeric transcriptional regulatory region comprises at least two copies or at least three copies of said enhancer.
17 . The method of claim 16 , wherein
a) the copies of said enhancer are immediately adjacent to one another; or,
b) at least one of said enhancers is in orientated in the forward or reverse orientation with respect to said promoter.
18 . The method of claim 14 , wherein said selectable marker encodes a polypeptide that confers tolerance to a herbicide.
19 . The method of claim 18 , wherein said selective agent comprises a herbicide.
20 . The method of anyone of claims 14 , wherein said cell comprises a plant cell.
21 . The method of claim 20 , wherein said plant cell is from a dicot.
22 . The method of claim 21 , wherein said dicot is soybean, wheat, canola, sunflower, cotton, or alfalfa.
23 . The method of claim 20 , wherein said plant cell is from a monocot.
24 . The method of claim 23 , wherein said monocot is maize, wheat, rice, barley, sorghum, or rye.
25 . The method of claim 14 , wherein said DNA construct comprises in the 5′ to 3′ or 3′ to 5′ orientation: the first polynucleotide of interest operably linked to a second heterologous promoter, operably linked to at least one of said enhancer domains, operably linked to the first heterologous promoter, operably linked to the selectable marker, wherein said first polynucleotide of interest and said selectable marker are expressed in divergent directions.
26 . A polynucleotide comprising a chimeric transcriptional regulatory element comprising a promoter that drives expression in a cell operably linked to at least one copy of a heterologous enhancer domain wherein said enhancer domain is selected from the group consisting of:
(a) the nucleotide sequence comprising SEQ ID NO: 1 or 10;
(b) the nucleotide sequence comprising at least 95% sequence identity to SEQ ID NO:1 or 17, wherein said polynucleotide has regulating transcriptional activity; and,
(c) the nucleotide sequence comprising at least 100 consecutive nucleotides of SEQ ID NO:1 or 17.
27 . The polynucleotide of claim 26 , wherein said enhancer domain does not comprise the sequence of SEQ ID NO:5.
28 . The polynucleotide of claim 26 , wherein said chimeric transcriptional regulatory region comprises at least two copies or at least three copies of said enhancer.
29 . The polynucleotide of claim 28 , wherein
a) the copies of said enhancer are immediately adjacent to one another;
b) at least one of said enhancers is in orientated in the forward or reverse orientation with respect to said promoter.
30 . An expression vector comprising the polynucleotide of claim 26 .
31 . A cell comprising the polynucleotide of claim 26 .
32 . The cell of claim 31 , wherein said cell is a plant cell.
33 . The cell of claim 32 , wherein said plant cell is from a dicot.
34 . The cell of claim 33 , where said dicot is soybean, wheat, canola, sunflower, cotton, or alfalfa.
35 . The cell of claim 32 , wherein said plant cell is from a monocot.
36 . The cell of claim 35 , wherein said monocot is maize, wheat, rice, barley, sorghum, or rye.
37 . A plant comprising the polynucleotide of claim 26 .
38 . The plant of claim 37 , wherein said plant is a dicot.
39 . The plant of claim 38 , where said dicot is soybean, wheat, canola, sunflower, cotton, or alfalfa.
40 . The plant of claim 37 , wherein said plant is a monocot.
41 . The plant of claim 40 , wherein said monocot is maize, wheat, rice, barley, sorghum, or rye.
42 . A method for improving transformation efficiency, increasing a single copy integration event, or increasing transformation efficacy of a plant comprising:
a) introducing in the plant a cassette comprising a chimeric transcriptional regulatory region comprising at least one enhancer domain operably linked to a heterologous promoter, said chimeric transcriptional regulatory region operably linked to a selectable marker wherein said enhancer domain is selected from the group consisting of:
i) the nucleotide sequence comprising SEQ ID NO:1 or 17;
ii) the nucleotide sequence comprising at least 95% sequence identity to SEQ ID NO:1 or 17, wherein said polynucleotide has regulating transcriptional activity; and,
iii) the nucleotide sequence comprising at least 100 consecutive nucleotides of SEQ ID NO:1 or 17;
b) contacting said plant with an effective concentration of an appropriate selection agent; and,
c) selecting the plant expressing said selectable marker.
43 . The method of claim 42 , wherein said enhancer does not comprise the sequence set forth in SEQ ID NO:5.
44 . The method of claim 42 , wherein said chimeric transcriptional regulatory region comprises at least two copies or at least three copies of said enhancer.
45 . The method of claim 44 , wherein
a) the copies of said enhancer are immediately adjacent to one another;
b) at least one of said enhancers is in orientated in the forward or reverse orientation with respect to said promoter.
46 . The method of claim 42 , wherein said cassette further comprises a polynucleotide of interest.
47 . The method of claim 42 , wherein said selectable marker confers tolerance to a herbicide.