Process for producing zinc oxide-protein complex
View Patent ↗An object of the present invention is to obtain a zinc oxide-protein complex which can be a source of nanoparticles of zinc oxide utilizing a protein having a cavity inside thereof. The process for producing a zinc oxide-protein complex according to the present invention includes a hydrogen peroxide addition step for adding hydrogen peroxide so that the concentration would be 60 mM or greater and 150 mM or less to a buffer containing a protein having a cavity inside thereof such as ferritin, zinc ion, and ammonia.
1. A process for producing a zinc oxide-protein complex comprising:
the step a) of preparing a buffer containing a protein having a cavity inside thereof, zinc ion, and ammonia, wherein the protein having a cavity inside thereof is ferritin; and
a step b) of adding hydrogen peroxide to the buffer so that the concentration of said hydrogen peroxide is 60 mM or greater and 150 mM or less.
2. The process for producing a zinc oxide-protein complex according to claim 1 , further comprising a step of
adding zinc acetate to the buffer prior to the hydrogen peroxide addition step.
3. The process for producing a zinc oxide-protein complex according to claim 2 wherein the concentration of zinc ion after adding the hydrogen peroxide is 1 mM or greater and 20 mM or less.
4. The process for producing a zinc oxide-protein complex according to claim 1 , further comprising a step of adding zinc nitrate to the buffer prior to the hydrogen peroxide addition step.
5. The process for producing a zinc oxide-protein complex according to claim 1 wherein the concentration of ammonia after adding the hydrogen peroxide is 8 mM or greater and 360 mM or less.
6. The process for producing a zinc oxide-protein complex according to claim 1 wherein said buffer is a HEPES buffer.
7. The process for producing a zinc oxide-protein complex according to claim 1 wherein said buffer has a pH of 6.9 or greater and 10.4 or less.
8. The process for producing a zinc oxide-protein complex according to claim 1 wherein said ammonia is coordinated with a zinc ion.
9. The process for producing a zinc oxide-protein complex according to claim 8 wherein water is further coordinated with the zinc ion.