Biosensor
It is an object of the present invention to provide a biosensor with an improved recovery yield of a test substance interacting with a physiologically active substance. The present invention provides a biosensor having a flow channel formed on a substrate, which is composed of a detection surface for detecting the interaction of a physiologically active substance with a test substance and a non-detection surface that does not detect said interaction, wherein the detection surface and non-detection surface are modified in such a way that the physiologically active substance can be immobilized thereon.
1 . A biosensor having a flow channel formed on a substrate, which is composed of a detection surface for detecting the interaction of a physiologically active substance with a test substance and a non-detection surface that does not detect said interaction, wherein the detection surface and non-detection surface are modified in such a way that the physiologically active substance can be immobilized thereon.
2 . The biosensor according to claim 1 wherein the detection surface and non-detection surface of a flow channel are modified with a polymer compound.
3 . The biosensor according to claim 2 wherein the polymer compound is a hydrophobic polymer.
4 . The biosensor according to claim 2 wherein the polymer compound is a hydrophilic polymer.
5 . The biosensor according to claim 1 which further has a mechanism for recovering a substance interacting with a physiologically active substance.
6 . The biosensor according to claim 1 wherein the substrate is composed of a metal surface or metal film.
7 . The biosensor according to claim 1 wherein the metal surface or metal film consists of a free electron metal selected from the group consisting of gold, silver, copper, platinum, and aluminum.
8 . The biosensor according to claim 1 which is used in non-electrochemical detection.
9 . The biosensor according to claim 1 which is used in surface plasmon resonance analysis.
10 . A method for producing the biosensor of claim 1 , which comprises a step of modifying the detection surface and non-detection surface of a flow channel in such a way that a physiologically active substance can be immobilized thereon.
11 . The method according to claim 10 wherein the detection surface and non-detection surface of a flow channel are modified with a polymer compound.
12 . The method according to claim 11 wherein the polymer compound is a hydrophobic polymer.
13 . The method according to claim 11 wherein the polymer compound is a hydrophilic polymer.
14 . A method for detecting or measuring a substance interacting with a physiologically active substance, which comprises the steps of: allowing the biosensor of claim 1 to come into contact with a physiologically active substance, so as to allow the said physiologically active substance to bind to the detection surface and non-detection surface of the flow channel of said biosensor via a covalent bond; and allowing a test substance to come into contact with the biosensor, to the detection surface and non-detection surface of the flow channel of which the physiologically active substance has been bound via a covalent bond.
15 . The method according to claim 14 wherein the step of allowing a physiologically active substance to bind to a biosensor, and the step of allowing a test substance to come into contact with the biosensor so as to detect or measure a substance interacting with the physiologically active substance, are carried out using different devices.
16 . The method according to claim 14 wherein a substance interacting with the physiologically active substance is detected or measured by non-electrochemical detection.
17 . The method according to claim 14 wherein a substance interacting with the physiologically active substance is detected or measured by surface plasmon resonance analysis.
18 . A method for analyzing a substance interacting with a physiologically active substance, which comprises identifying and recovering a substance interacting with a physiologically active substance using the biosensor of claim 1 , and determining the structure of the recovered substance using a mass spectrometer.