Array for rapid detection of a microorganism
View Patent ↗A method for detecting a microorganism or class of microorganisms is provided. More specifically, the method employs an array that contains a plurality of discrete regions (referred to as “addresses”) spaced apart on a solid support in a predetermined pattern. The addresses are selected so that the array provides a distinct spectral response (e.g., pattern of colors) or “fingerprint” for a particular microorganism or class of microorganisms. For example, the array may provide a certain spectral response in the presence of one microorganism or class of microorganisms (e.g., gram-negative bacteria), but provide a completely different spectral response in the presence of another microorganism or class of microorganisms (e.g., gram-positive bacteria). Detection of the spectral response provided by the array may thus allow for differentiation between microorganisms.
1. A method for detecting a microorganism in a sample, the method comprising:
contacting the sample with an array, the array comprising a plurality of individual array addresses spaced apart in a predetermined pattern on a solid support, wherein the addresses each contain a colorant so that the array produces a visually observable spectral response;
detecting the spectral response; and
correlating the detected spectral response to the presence of one or more microorganisms.
2. The method of claim 1 , wherein one or more of the addresses contain a pH-sensitive colorant.
3. The method of claim 2 , wherein the pH sensitive colorant is a phthalein, hydroxyanthraquinone, arylmethane, aromatic azo, or a derivative thereof.
4. The method of claim 1 , wherein one or more of the addresses contain a metal complexing colorant.
5. The method of claim 1 , wherein one or more of the addresses contain a solvatochromoatic colorant.
6. The method of claim 1 , wherein the array contains from 2 to 50 individual array addresses.
7. The method of claim 1 , wherein the array contains from 3 to 40 individual array addresses.
8. The method of claim 1 , wherein at least two of the addresses are spaced apart a distance of from about 0.01 to about 100 millimeters.
9. The method of claim 1 , wherein at least two of the addresses are spaced apart a distance of from about 0.1 to about 50 millimeters.
10. The method of claim 1 , wherein the spectral response is correlated to the presence of one or more microorganisms at a concentration of about 1×10 3 or more colony forming units per milliliter of the sample.
11. The method of claim 1 , wherein the spectral response is correlated to the presence of one or more microorganisms at a concentration of about 1×10 6 or more colony forming units per milliliter of the sample.
12. The method of claim 1 , wherein the colorant is applied to the solid support in the form of a composition that comprises a carrier.
13. The method of claim 12 , wherein the colorant constitutes from about 0.1 to about 100 milligrams per milliliter of the carrier.
14. The method of claim 12 , wherein the colorant constitutes from about 0.5 to about 60 milligrams per milliliter of the carrier.
15. The method of claim 1 , wherein the array is printed onto the support.
16. The method of claim 1 , wherein the spectral response is detected and compared to a control array.
17. The method of claim 1 , wherein the spectral response is visually detected.
18. The method of claim 1 , wherein the spectral response is produced in about 30 minutes or less after the array is contacted with the sample.
19. The method of claim 1 , wherein the spectral response is produced in about 5 minutes or less after the array is contacted with the sample.
20. The method of claim 1 , wherein the microorganism is a fungus.
21. The method of claim 20 , wherein the fungus is C. albicans, A. pullulans, P. janthinellum , or a mixture thereof.
22. The method of claim 1 , wherein the microorganism is a bacteria.
23. The method of claim 22 , wherein the bacteria is a gram-positive bacteria.
24. The method of claim 23 , wherein the gram-positive bacteria is S. aureus, L. acidophilus, S. epidermidis, B. subtilis, E. faecalis, S. pyogenes, S. pneumoniae , or a mixture thereof.
25. The method of claim 22 , wherein the bacteria is a gram-negative bacteria.
26. The method of claim 25 , wherein the gram-negative bacteria is E. coli, P. aeruginosa, K. pneumoniae, P. mirabilis, M. lacunata, H. influenzae , or a mixture thereof.