Compounds capable of inhibiting HIV-1 infection
View Patent ↗This invention provides an antibody capable of specifically inhibiting the fusion of an HIV-1 envelope glycoprotein + cell with an appropriate CD4 + cell without cross reacting with the HIV-1 envelope glycoprotein or CD4 and capable of inhibiting infection by one or more strains of HIV-1. This antibody is then used to identify a molecule which is important for HIV infection. Different uses of the antibody and the molecule are described.
1. A monoclonal antibody or a portion of such monoclonal antibody effective to (a) specifically inhibit 67% or greater of fusion of a CD4+ PM-1 cell to a HeLa cell expressing envelope glycoprotein from HIV-1 JR-FL , and (b) inhibit 18% or less of fusion of a CD4+ SUP-T1 cell to a HeLa cell expressing envelope protein from HIV-1 LAI , wherein the antibody (i) does not crossreact with HIV-1 envelope glycoprotein or CD4, (ii) reacts with an antigen on the surface of a PM-1 cell having an approximate molecular weight of 44 kD, (iii) does not react with an antigen on the surface of a SUP-T1 cell, and (iv) is at least as active as monoclonal antibody PA-7 produced by the hybridoma designated PA-7 (ATCC Accession No. PTA-6638) in inhibiting fusion as recited in (a) above and less active than inonoclonal antibody PA-6 produced by the hybridoma designated PA-6 (ATCC Accession No. PTA-6637) in inhibiting fusion as recited in (b) above.
2. The monoclonal antibody of claim 1 , wherein the monoclonal antibody is a chimeric monoclonal antibody.
3. The monoclonal antibody of claim 1 , wherein the monoclonal antibody is a humanized monoclonal antibody.
4. The monoclonal antibody of claim 1 , wherein the monoclonal antibody is a human monoclonal antibody.
5. The monoclonal antibody of claim 1 , wherein the portion of the monoclonal antibody is a single chain antibody or an antigen binding fragment.
6. The monoclonal antibody of claim 1 or the portion of such monoclonal antibody, wherein the monoclonal antibody or the portion of such monoclonal antibody is labeled with a detectable marker.
7. The monoclonal antibody of claim 6 or the portion of such monoclonal antibody, wherein the detectable marker is a radioactive isotope, an enzyme, a dye or biotin.