Busulfan immunoassay
View Patent ↗Novel conjugates of busulfan and novel busulfan immunogens derived from α-substituted derivatives of busulfan and antibodies generated by these busulfan linked immunogens are useful in immunoassays for the quantification and monitoring of busulfan in biological fluids.
1. A compound of the formula:
wherein A is lower alkylene;
X′ is a functional linking group;
Y is an organic spacing group;
X is a terminal functional group capable of reacting with a polyamine polymer;
p and z are independent integers of from 0 to 1.
2. The compound of claim 1 , wherein X is
wherein R 3 is hydrogen or taken together with its attached oxygen atom forms a reactive ester and R 4 is oxygen or sulfur.
3. The compound of claim 2 , wherein X is
and R 3 is hydrogen.
4. The compound of claim 3 , wherein X is
and R 3 forms a reactive ester.
5. The compound of claim 4 , wherein the ester formed is a lower alkyl ester, imidoester or amidoester.
6. The compound of claim 5 , wherein z is 0 and p is 1.
7. The compound of claim 6 , wherein A is —(CH 2 ) v — and v is 1 or 2.
8. The compound of claim 7 , wherein v is 2.
9. A conjugate of a carrier with a compound of the formula:
wherein A is lower alkylene;
X′ is a functional linking group;
Y is an organic spacing group;
X is a terminal functional group capable of reacting with a polyamine polymer;
p and z are independent integers of from 0 to 1 wherein said polyamine polymer reacts with X to form said conjugate;
or a compound of the formula
wherein A′ is lower alkylene or lower alkenylene;
X′, Y, X and z are as above.
10. The conjugate of a polyamine polymer with claim 9 , wherein said compound is
wherein A, X′, Y, X, p and z are as above.
11. The conjugate of claim 10 , wherein z is 0.
12. The conjugate of claim 11 , wherein X is
wherein R 3 is hydrogen or taken together with its attached oxygen atom forms a reactive ester and R 4 is oxygen or sulfur.
13. The conjugate of claim 12 , wherein the carrier contains one or more amino groups linked by
wherein R 4 is oxygen or sulfur.
14. The conjugate of claim 13 , wherein A is —(CH 2 ) v — and v is an integer from 1 to 3.
15. The conjugate of claim 14 , wherein v is 2.
16. An immunogen containing an immunogenic polyamine polymer linked to a compound of the formula:
wherein A is lower alkylene;
X′ is a functional linking group;
Y is an organic spacing group;
X is a terminal functional group capable of reacting with a polyamine polymer;
p and z are independent integers of from 0 to 1 wherein said immunogenic polyamine polymer reacts with X to form said immunogen;
or a compound of the formula
wherein A′ is lower alkylene or lower alkenylene;
X′, Y, X and z are as above.
17. The immunogen of claim 16 , wherein the immunogenic polymer contains one or more amino groups linked by
wherein R 4 is oxygen or sulfur.