Optical system and method for optically analyzing light from a sample
View Patent ↗An optical system for analyzing light from a plurality of samples is provided. The optical system includes a plurality of holders adapted to have samples located therein, a collection lens, a transmission grating, and a reimaging lens. The collection lens is configured to receive and substantially collimate light from the samples. The transmission grating is configured to spectrally disperse the substantially collimated light from the collection lens. The reimaging lens is configured to receive the light from the light dispersing element and direct the light onto a light detection device. A method of optically analyzing at least one sample is also provided.
1. An optical system for analyzing light from a plurality of samples, comprising:
a plurality of capillaries, wherein the capillaries contain the plurality of samples and each sample in each capillary emits light in multiple colors during electrophoresis separation;
a collection lens configured to receive and substantially collimate the light from the plurality of capillaries;
a transmission grating configured to spectrally disperse the substantially collimated light from the collection lens; and
a reimaging lens configured to receive the spectrally dispersed light from the transmission grating and direct the light onto a light detection device,
wherein the system is configured to compensate for chromatic aberration in the spectrally dispersed light, the compensation comprising an optical axis of the reimaging lens being non-perpendicular with a surface of the light detection device.
2. The system of claim 1 , wherein the system further comprises the light detection device, and wherein said light detection device is a two-dimensional, multi-element photodetector having respectively perpendicular spatial and spectral detection axes.
3. The system of claim 2 , wherein light from the plurality of capillaries is spatially dispersed along the spatial detection axis of the photodetector, such that light from each capillary can be detected.
4. The system of claim 3 , wherein light from each capillary is spectrally dispersed along the spectral detection axis of the photodetector, such that the multiple colors from each capillary can be detected.
5. The system of claim 4 , wherein the system is configured to independently detect the multiple colors.
6. The system of claim 1 , further comprising an excitation light source for illuminating the plurality of capillaries.
7. The system of claim 6 , wherein light from the excitation light source does not pass through the collection lens prior to illuminating the plurality of capillaries.
8. The system of claim 7 , wherein the excitation light source is an LED.
9. The system of claim 1 , wherein the system further comprises the light detection device, and wherein the light detection device comprises a charge-coupled device (CCD).