Serum Prolactin Binding Protein in Epithelial Carcinoma
The present invention relates to antibodies that have specificity towards prolactin binding protein (PRLBP) that is either bound to a binding partner or unbound to a binding partner, as well as antibodies towards PRLBP regardless of the binding state of PRLBP. The present invention also provides methods of using these PRLBP-specific antibodies, such as method of diagnosing and monitoring the progression of diseases such as epithelial carcinomas, osteoporosis, infertility and cachexia.
1 . An antibody that has specificity towards prolactin binding protein that is both bound and unbound to a binding partner (total PRLBP).
2 . The antibody of claim 2 , wherein said antibody is a monoclonal antibody.
3 . The antibody of claim 3 , wherein said antibody is secreted by hybridoma 1A2B1.
4 . An antibody that has specificity towards prolactin binding protein that is bound to a binding partner (bPRLBP).
5 . The antibody of claim 4 , wherein said antibody does not have specificity towards PRLBP that is unbound to said binding partner.
6 . The antibody of claim 5 , wherein said antibody is a monoclonal antibody.
7 . The antibody of claim 6 , wherein said binding partner is selected from the group consisting of prolactin and growth hormone.
8 . The antibody of claim 7 , wherein said binding partner is prolactin.
9 . The antibody of claim 7 , wherein said antibody is secreted by hybridoma 1A2D6 and 1A8E7.
10 . An antibody that has specificity towards prolactin binding protein that is not bound to a binding partner (uPRLBP).
11 . The antibody of claim 10 , wherein said antibody does not have specificity towards PRLBP that is bound to said binding partner.
12 . The antibody of claim 11 , wherein said antibody is a monoclonal antibody.
13 . The antibody of claim 12 , wherein said antibody is secreted by hybridoma 1H6C11 and 4F3B3.
14 . A method of detecting prolactin binding protein (PRLBP) in a sample, said method comprising
a) contacting said sample with an antibody that is specific for said total PRLBP; and
b) detecting the binding of said antibody to said PRLBP.
15 . The method of claim 14 , wherein said sample is selected from the group consisting of mammary tissue, prostate tissue, testicular tissue, liver, blood, serum, plasma, milk, seminal plasma, and urine.
16 . The method of claim 15 , wherein said antibody is a monoclonal antibody.
17 . The method of claim 16 , wherein said monoclonal antibody is secreted by hybridoma 1A2B1.
18 . The method of claim 17 , wherein said detecting comprises a radioimmunoassay, an enzyme immunoassay, a spectrophotometric assay and an electrophoresis assay.