ENZYME INHIBITION USING NANOPARTICLES
Methods and compositions useful for the inhibition of enzyme activity, including in the treatment of enzymatic dermatitis, are provided. Such compositions comprise a composite material comprising: (a) an exfoliated nanoparticle having a surface and (b) a metal selected from Groups 3 to 12, aluminum and magnesium, wherein the metal is loaded onto the surface of the nanoparticle.
1 . A method of inhibiting the activity of an enzyme, comprising contacting an enzyme selected from the group consisting of lipases and proteases with an effective amount of a composite material comprising: (a) an exfoliated nanoparticle having a surface, and (b) a metal selected from Groups 3 to 12, aluminum and magnesium, wherein the metal is loaded onto the surface of the nanoparticle.
2 . The method of claim 1 , wherein the metal is selected from the group consisting of silver, copper, zinc, manganese, platinum, palladium, gold, calcium, barium, aluminum, iron, and mixtures thereof.
3 . The method of claim 1 , wherein the metal is silver.
4 . The method of claim 1 , wherein the nanoparticle comprises a nanoclay.
5 . The method of claim 1 , wherein the nanoparticle comprises exfoliated Laponite.
6 . The method of claim 1 , wherein the enzyme is lipase.
7 . A method of treating enzymatic dermatitis, comprising topically applying to an area of skin affected with enzymatic dermatitis an effective amount of a composite material comprising: (a) an exfoliated nanoparticle having a surface and (b) a metal selected from Groups 3 to 12, aluminum and magnesium, wherein the metal is loaded onto the surface of the nanoparticle.
8 . The method of claim 7 , wherein the metal is selected from the group consisting of silver, copper, zinc, manganese, platinum, palladium, gold, calcium, barium, aluminum, iron, and mixtures thereof.
9 . The method of claim 7 , wherein the metal is silver.
10 . The method of claim 7 , wherein the nanoparticle comprises a nanoclay.
11 . The method of claim 7 , wherein the nanoparticle comprises exfoliated Laponite.
12 . The method of claim 7 , wherein said composite material is in the form of a solution.
13 . The method of claim 7 , wherein said composite material is in the form of a solid.
14 . The method of claim 7 , wherein said composite material is in the form of a gel.
15 . The method of claim 7 , further comprising an antibiotic (or antimicrobial?).
16 . The method of claim 7 , further comprising an anti-irritant.
17 . The method of claim 7 , further comprising an antifungal.