Modified ethylenediamine-N, N′-disuccinate: ethylenediamine lyase
View Patent ↗The present invention provides a modified ethylenediamine-N,N′-disuccinate:ethylenediamine lyase. The present invention also provides a protein that comprises the amino acid sequence represented by SEQ ID NO: 1; or a protein that comprises an amino acid sequence derived from the amino acid sequence represented by SEQ ID NO: 1 by deletion, substitution, or addition of one or more amino acid residues, and has an ethylenediamine-N,N′-disuccinate:ethylenediamine lyase activity.
1. An isolated protein comprising the amino acid sequence of SEQ ID NO: 1, and having an ethylenediamine-N,N′-disuccinate:ethylenediamine lyase activity.
2. An isolated protein comprising the amino acid sequence of SEQ ID NO:1 having 1 to 10 substitutions, wherein one substitution is the substitution of the lysine residue at position 120 with a glutamic acid residue, and wherein said protein has ethylenediamine-N,N′-disuccinate:ethylenediamine lyase activity that is heat resistant.
3. An isolated protein comprising the amino acid sequence of SEQ ID NO:1 having 1 to 10 substitutions, wherein one substitution is the substitution of the isoleucine residue at position 166 with a serine residue, and wherein said protein has ethylenediamine-N,N′-disuccinate:ethylenediamine lyase activity that is heat resistant.
4. An isolated protein comprising the amino acid sequence of SEQ ID NO:1 having 1 to 10 substitutions, wherein one substitution is the substitution of the isoleucine residue at position 166 with a threonine residue, and wherein said protein has ethylenediamine-N,N′-disuccinate:ethylenediamine lyase activity that is heat resistant.
5. An isolated protein comprising the amino acid sequence of SEQ ID NO:1 having 1 to 10 substitutions, wherein one substitution is the substitution of the alanine residue at position 365 with a valine residue, and wherein said protein has ethylenediamine-N,N′-disuccinate:ethylenediamine lyase activity that is heat resistant.
6. An isolated protein comprising the amino acid sequence of SEQ ID NO:1 having 2 to 10 substitutions, wherein said substitutions include the substitution of the lysine residue at position 120 with a glutamic acid residue and the substitution of the isoleucine residue at position 166 with a serine residue, and wherein said protein has ethylenediamine-N,N′-disuccinate:ethylenediamine lyase activity that is heat resistant.
7. An isolated protein comprising the amino acid sequence of SEQ ID NO:1 having 2 to 10 substitutions, wherein said substitutions include the substitution of the lysine residue at position 120 with a glutamic acid residue and the substitution of the isoleucine residue at position 166 with a threonine residue and wherein said protein has ethylenediamine-N,N′-disuccinate:ethylenediamine lyase activity that is heat resistant.
8. An isolated protein comprising the amino acid sequence of SEQ ID NO:1 having 2 to 10 substitutions, wherein said substitutions include the substitution of the isoleucine residue at position 166 with a serine residue, and the substitution of the alanine residue at position 365 with a valine residue, and wherein said protein has ethylenediamine-N,N′-disuccinate:ethylenediamine lyase activity that is heat resistant.
9. An isolated protein comprising the amino acid sequence of SEQ ID NO:1 having 2 to 10 substitutions, wherein said substitutions include the substitution of the isoleucine residue at position 166 with a threonine residue, and the substitution of the alanine residue at position 365 with a valine residue, and wherein said protein has ethylenediamine-N,N′-disuccinate:ethylenediamine lyase activity that is heat resistant.
10. An isolated protein comprising the amino acid sequence of SEQ ID NO:1 having 3 to 10 substitutions, wherein said substitutions include the substitution of the lysine residue at position 120 with a glutamic acid residue, and the substitution of the isoleucine residue at position 166 with a serine residue and the substitution of the alanine residue at position 365 with a valine residue, and wherein said protein has ethylenediamine-N,N′-disuccinate:ethylenediamine lyase activity that is heat resistant.
11. An isolated protein comprising the amino acid sequence of SEQ ID NO:1 having 3 to 10 substitutions, wherein said substitutions include the substitution of the lysine residue at position 120 with a glutamic acid residue and the substitution of the isoleucine residue at position 166 with a threonine residue and the substitution of the alanine residue at position 365 with a valine residue, and wherein said protein has ethylenediamine-N,N′-disuccinate:ethylenediamine lyase activity that is heat resistant.
12. The isolated protein according to claim 2 , wherein the ethylenediamine-N,N′-disuccinate:ethylenediamine lyase retains its activity in the temperature range of 45° C. to 60° C.