Process and genes for expression and overexpression of active [FeFe] hydrogenases
View Patent ↗A process for expression of active [FeFe]-hydrogenase in a host organism that does not contain either the structural gene(s) for [FeFe]-hydrogenases and/or homologues for the maturation genes HydE, HydF and HyG, comprising: cloning the structural hydrogenase gene(s) and/or the maturation genes HydE, HydF and HydG from an organisms that contains these genes into expression plasmids; transferring the plasmids into an organism that lacks a native [FeFe]-hydrogenase or that has a disrupted [FeFe]-hydrogenase and culturing it aerobically; and inducing anaerobiosis to provide [FeFe] hydrogenase biosynthesis and H?2#191 production.
1. An E. coli cell that co-expresses exogenous genes encoding HydEF and HydG and also expresses a hydrogenase structural gene.
2. The E. coli cell of claim 1 , wherein the HydEF and HydG genes are from a green algae.
3. The E. coli cell of claim 1 , wherein the HydEF and HydG genes are from an organism of the genus Chlamydomonas.
4. The E. coli cell of claim 1 , wherein the HydEF and HydG genes are from Chlamydomonas reinhardtii.
5. The E. coli cell of claim 1 , wherein the hydrogenase structural gene is HydA1 or HydA2.
6. The E. coli cell of claim 5 , wherein the HydA1 or HydA2 gene is from a green algae.
7. The E. coli cell of claim 5 , wherein the HydA1 or HydA2 gene is from an organism of the genus Chlamydomonas.
8. The E. coli cell of claim 5 , wherein the HydA1 or HydA2 gene is from Chlamydomonas reinhardtii.