Compositions and methods for making and modifying oils
View Patent ↗The invention provides novel methods for making or modifying oils, e.g., plant, animal or microbial oils, such as vegetable oils or related compounds, that are low in a particular fatty acid(s), for example, low linoleic oils, low linolenic oils, low palmitic oils, low stearic oils or oils low in a combination thereof.
1. A method for making an oil or a glyceride low in palmitic acid comprising:
(A) contacting an oil or a glyceride comprising palmitic acid with a polypeptide as set forth in SEQ ID NO:924 wherein the polypeptide selectively hydrolyzes palmitic acid; and
(B) removing the hydrolyzed palmitic acid from the oil or glyceride.
2. The method of claim 1 , wherein the hydrolyzed palmitic acid is removed from the oil or the glyceride by a process selected from a group consisting of: a steam distillation, a saponification reaction, or use of a silica.
3. The method of claim 1 , wherein the polypeptide is added to the oil or the glyceride before a degumming step, during a degumming step, after a degumming step, or a combination thereof.
4. The method of claim 1 , wherein the oil or glyceride comprise: a plant oil, an animal oil, a microbial oil, or a restaurant grease.
5. The method of claim 4 , where the plant oil is selected from: a vegetable oil, a soy oil, a canola oil, a palm oil, a cottonseed oil, a com oil, a palm kernel-derived oil, a rice bran oil, a coconut oil, a peanut oil, asesame oil, a sunflower oil, a fruit seed oil, a grapeseed oil, an apricot oil, a borage oil, or an algae oil.
6. The method of claim 4 , wherein the animal oil is selected from: a fish oil; an animal processing by-product; or an animal feed bypass fat.
7. The method of claim 1 , wherein the oil or glyceride is an impure or mixed source of a plant oil, an animal, a microbial oil, or a combination thereof.
8. The method of claim 1 , wherein the contacting reaction conditions comprising a pH in the range from about 4 to about 10.
9. The method of claim 1 , wherein the contacting conditions comprise higher water conditions for the selective hydrolysis reaction.
10. The method of claim 1 , where the polypeptide hydrolyzes all of the palmitic acid in the oil or the glyceride, thereby producing an oil or a glyceride completely lacking palmitic acid.
11. The method of claim 1 , wherein the oil or the glyceride further comprises: a glycolipid, a phospholipid, a sphingolipid, a coenzyme A, an oxidized lipid or an ether lipid, a linoleic acid (cis-9, cis-12-octadecadienoic acid), a linolenic acid, a stearic acid, a saturated fatty acid, a fatty acid comprising a butyric acid, valeric acid, caproic acid, caprylic acid, pelargonic acid, capric acid, lauric acid, myristic acid, palmitic acid, margaric (daturic) acid, stearic acid,-arachidic acid, behenic acid, lignoceric acid or cerotic acid, a monoenoic fatty acid; a lipid comprising an obtusilic acid, caproleic acid, lauroleic acid, linderic acid, myristoleic acid, physeteric acid, tsuzuic acid, palmitoleic acid, petroselinic acid or oleic acid, a lipid comprising a polyenoic fatty acid (polyunsaturated fatty acid, or PUFA), a polyenoic fatty acid comprising an eicosapentaenoic acid, linoleic acid, γ-linolenic acid, dihomo-γ-linolenic acid, arachidonic acid, 7,10,13,16-docosatetraenoic acid, 4,7,10,13,16-docosapentaenoic acid, oc-linolenic acid (9,12, 15- octadecatrienoic acid), stearidonic acid, 8,11,14,17-eicosatetraenoic acid, 5,8,11,14,17-eicosapentaenoic acid (EPA), 7,10,13,16,19-docosapentaenoic (DPA), 4,7,10,13,16,19- docosahexaenoic acid (DHA), or 5,8,11-eicosatrienoic, a branched chain fatty acid, a branched methoxy fatty acid, a ring-containing fatty acid, an acetylenic fatty acid, a hydroxy fatty acid, a fatty acid amide, a keto fatty acid, or a halogenated fatty acid.
12. The method of claim 1 , wherein the polypeptide encoded by a nucleic acid sequence as set forth in SEQ ID NO:923.