Process for producing L-arginine, L-ornithine or L-citrulline
View Patent ↗The present invention provides a polypeptide which has: (i) an amino acid sequence wherein one or more amino acid residues are substituted in the region at positions 20 to 38 from the N terminus of the amino acid sequence shown in SEQ ID NO: 1; or (ii) an amino acid sequence wherein one or more amino acid residues are substituted in the region at positions 20 to 38 from the N terminus of the amino acid sequence shown in SEQ ID NO: 1 and one or more amino acid residues are deleted, substituted or added in the region at positions 1 to 19 or 39 to 294 ; and which has N-acetylglutamate kinase activity.
1. A process for producing L-arginine, L-ornithine or L-citrulline, comprising the steps of:
culturing in a medium an isolated microorganism carrying a DNA comprising a nucleotide sequence selected from the group consisting of SEQ ID NOS: 4, 6, 8, 10 and 12;
allowing L-arginine, L-ornithine or L-citrulline to form and accumulate in the medium; and
recovering L-arginine, L-ornithine or L-citrulline from the medium.
2. A process for producing L-arginine, L-ornithine or L-citrulline, comprising the steps of:
culturing in a medium an isolated microorganism carrying a DNA which hybridizes with DNA consisting of a nucleotide sequence complementary to the nucleotide sequence of DNA encoding SEQ ID NO: 1 at 65° C. in 0.7-1.0 mol/l sodium chloride followed by washing at 65° C. with 0.1-2 ×SSC solution, said DNA being selected from the group consisting of the following (i) to (iii):
(i) DNA encoding a polypeptide wherein the amino acid residue corresponding to the residue at position 26 from the N terminus of the amino acid sequence shown in SEQ ID NO: 1 is an amino acid residue other than L-alanine;
(ii) DNA encoding a polypeptide wherein the amino acid residue corresponding to the residue at position 31 from the N terminus of the amino acid sequence shown in SEQ ID NO: 1 is an amino acid residue other than L-methionine; and
(iii) DNA encoding a polypeptide wherein the amino acid residue corresponding to the residue at position 26 from the N terminus of the amino acid sequence shown in SEQ ID NO: 1 is an amino acid residue other than L-alanine and the amino acid residue corresponding to the residue at position 31 is an amino acid residue other than L-methionine, and
which encodes a polypeptide having N-acetylglutamate kinase activity;
allowing L-arginine, L-ornithine or L-citrulline to form and accumulate in the medium; and
recovering L-arginine, L-ornithine or L-citrulline from the medium.
3. The process according to claim 2 , wherein the amino acid residue corresponding to the residue at position 26 from the N terminus of the amino acid sequence shown in SEQ ID NO: 1 is L-valine, L-leucine or L-isoleucine and the amino acid residue corresponding to the residue at position 31 is L-valine.
4. A process for producing L-arginine, L-ornithine or L-citrulline, comprising the steps of:
culturing in a medium an isolated microorganism carrying a DNA which hybridizes with DNA consisting of a nucleotide sequence complementary to the nucleotide sequence of DNA encoding SEQ ID NO: 1 at 65° C. in 0.7-1.0 mol/l sodium chloride followed by washing at 65° C with 0.1-2 ×SSC solution, selected from the group consisting of the following (i) to (iii):
(i) DNA having a nucleotide sequence wherein the region corresponding to the region at positions 76 to 78 from the 5′ end of the nucleotide sequence shown in SEQ ID NO: 2 is guanine-thymidine-thymidine, cytosine-thymidine-guanine or adenine-thymidine-cytosine;
(ii) DNA having a nucleotide sequence wherein the region corresponding to the region at positions 91 to 93 from the 5′ end of the nucleotide sequence shown in SEQ ID NO: 2 is guanine-thymidine-guanine; and
(iii) DNA having a nucleotide sequence wherein the region corresponding to the region at positions 76 to 78 from the 5′ end of the nucleotide sequence shown in SEQ ID NO: 2 is guanine-thymidine-thymidine, cytosine-thymidine-guanine or adenine-thymidine-cytosine, and the region corresponding to the region at positions 91 to 93 is guanine-thymidine-guanine; and
which encodes a polypeptide having N-acetylglutamate kinase activity;
allowing L-arginine, L-ornithine or L-citrulline to form and accumulate in the medium; and
recovering L-arginine, L-ornithine or L-citrulline from the medium.
5. A process according to any one of claims 1 - 4 , wherein said DNA is a recombinant DNA obtained by incorporating said DNA into a vector.
6. The process according to claim 5 , wherein the transcriptional repression activity of the arginine repressor on the arginine operon is reduced or lost.
7. The process according to claim 6 , wherein ornithine carbamoyl transferase activity is reduced or lost.
8. The process according to claim 7 , wherein argininosuccinate synthase activity is reduced or lost.
9. The process according to claim 8 , wherein the microorganism belonging belongs to the genus Corynebacterium.
10. The process according to claim 9 , wherein the microorganism is Corynebacterium glutamicum.