siRNA targeting ras-related nuclear protein
View Patent ↗Efficient sequence specific gene silencing is possible through the use of siRNA technology. By selecting particular siRNAs by rational design, one can maximize the generation of an effective gene silencing reagent, as well as methods for silencing genes. Methods, compositions, and kits generated through rational design of siRNAs are disclosed.
1. An siRNA molecule, wherein said siRNA molecule consists of: (a) a sense region and an antisense region, wherein said sense region and said antisense region together form a duplex region consisting of 19-30 base pairs and said antisense region comprises a sequence that is the complement of SEQ ID NO: 449; and (b) either no overhang regions or one or more overhang regions, wherein each overhang region is six or fewer nucleotides in length.
2. The siRNA molecule of claim 1 , wherein said antisense region and said sense region are each 19-25 bases in length.
3. The siRNA molecule of claim 2 , wherein said antisense region and said sense region are each 19 bases in length.
4. The siRNA molecule of claim 1 , wherein said siRNA molecule has one or more overhang regions.
5. The siRNA molecule of claim 1 , wherein said siRNA molecule has no overhang regions.
6. The siRNA molecule of claim 2 , wherein said siRNA molecule has one or more overhang regions.
7. The siRNA molecule of claim 2 , wherein said siRNA molecule has no overhang regions.
8. The siRNA molecule of claim 3 , wherein said siRNA molecule has one or more overhang regions.
9. The siRNA molecule of claim 3 , wherein said siRNA molecule has no overhang regions.
10. A chemically synthesized double stranded siRNA molecule, wherein:
(a) each strand of said siRNA molecule is between 19 and 30 nucleotides in length; and
(b) strand of said siRNA molecule comprises a sequence that is the complement of SEQ ID NO: 449.
11. The chemically synthesized double stranded siRNA molecule of claim 10 , wherein each strand of said siRNA molecule is 19 nucleotides in length.
12. A pool of at least two siRNAs, wherein said pool comprises a first siRNA and a second siRNA, wherein said first siRNA consists of: (a) a first sense region and a first antisense region, wherein said first sense region and said first antisense region together form a first duplex region consisting of 19-30 base pairs and said first antisense region comprises a sequence that is the complement of SEQ ID NO: 449; and (b) either no overhang regions or one or more overhang regions, wherein each overhang region is six or fewer nucleotides in length, and said second siRNA consists of: (a) a second sense region and a second antisense region, wherein said second sense region and said second antisense region together form a second duplex region consisting of 19-30 base pairs and said second antisense region comprises a sequence that is the complement of SEQ ID NO: 458; and (b) either no overhang regions or one or more overhang regions, wherein each overhang region is six or fewer nucleotides in length.
13. The pool of claim 12 , wherein said first siRNA and said second siRNA each has no overhang regions.
14. The pool of claim 12 , wherein said first duplex region and said second duplex region are each 19 -25 base pairs in length.
15. The pool of claim 14 , wherein said first duplex region and said second duplex region are each 19 base pairs in length.
16. The pool of claim 15 , wherein the first duplex region contains no overhang regions.
17. The pool of claim 15 , wherein the second duplex region contains no overhang regions.
18. The pool of claim 17 , wherein the first duplex region contains one or more overhang regions.
19. The pool of claim 16 , wherein the second duplex region contains one or more overhang regions.
20. The chemically synthesized double stranded siRNA molecule of claim 10 , wherein each strand of said siRNA molecule is 19 -25 nucleotides in length.