Taxol immunoassay
Novel conjugates of taxol and novel taxol immunogens derived from the 9 and 7 positions of taxol and monoclonal antibodies generated by these taxol linked immunogens are useful in immunoassays for the quantification and monitoring of taxol in biological fluids.
1 . A compound of the formula:
Y is an organic spacing group;
X is a functional terminal group capable of binding to a polyamine polypeptide; and
p is an integer from 0 to 1.
2 . The compound of claim 1 , wherein p is 0.
3 . The compound of claim 2 , wherein X is
wherein R 3 is hydrogen or taken together with its attached oxygen atom forms a reactive ester and R 4 is oxygen or sulfur.
4 . The compound of claim 3 , wherein X is
and R 3 is hydrogen.
5 . The compound of claim 3 , wherein X is
and R 3 forms a reactive ester.
6 . The compound of claim 5 , wherein the ester formed is a lower alkyl ester, imidoester or amidoester.
7 . The compound of claim 1 , wherein p is 1.
8 . The compound of claim 7 , wherein Y is alkylene containing from 1 to 10 carbon atoms,
wherein n and o are integers from 0 to 6, and m is an integer from 1 to 6.
9 . A compound of the formula:
wherein Y is an organic spacing group;
X is a functional terminal group capable of binding to a polyamine
polypeptide; and B is —CH 2 — or
or mixtures thereof.
10 . The compound of claim 9 , wherein p is 0.
11 . The compound of claim 10 , wherein X is
wherein R 3 is hydrogen or taken together with its attached oxygen atom forms a reactive ester and R 4 is oxygen or sulfur.
12 . The compound of claim 11 , wherein X is
and R 3 is hydrogen.
13 . The compound of claim 12 , wherein X is
and R 3 forms a reactive ester.
14 . The compound of claim 13 , wherein the ester formed is a lower alkyl ester, imidoester or amidoester.
15 . The compound of claim 14 , wherein p is 1.
16 . The compound of claim 15 , wherein X is
wherein R 3 is hydrogen or taken together with its attached oxygen atom forms a reactive ester and R 4 is oxygen or sulfur.
17 . The compound of claim 16 , wherein Y is alkylene containing from 1 to 10 carbon atoms,
wherein n and o are integers from 0 to 6, and m is an integer from 1 to 6.
18 . A conjugate of a carrier with a compound of the formula:
Y is an organic spacing group;
X is a functional terminal group capable of binding to a polyamine polypeptide; and
p is an integer from 0 to 1.
19 . The conjugate of claim 18 , wherein p is 0.
20 . The conjugate of claim 19 , wherein X is
wherein R 3 is hydrogen or taken together with its attached oxygen atom forms a reactive ester and R 4 is oxygen or sulfur.
21 . The conjugate of claim 19 , wherein p is 1 and Y is alkylene containing from 1 to 10 carbon atoms,
wherein n and o are integers from 0 to 6, and m is an integer from 1 to 6.
22 . The conjugate of claim 21 , wherein the carrier contains one or more amino groups linked by
wherein R 4 is oxygen or sulfur.
23 . A compound of the formula:
wherein X, Y and p are as above and B is —CH 2 — or
or mixtures thereof.
24 . The compound of claim 23 , wherein p is 0.
25 . The compound of claim 24 , wherein X is
wherein R 3 is hydrogen or taken together with its attached oxygen atom forms a reactive ester and R 4 is oxygen or sulfur.
26 . The compound of claim 23 , wherein p is 1.
27 . The compound of claim 26 , wherein Y is alkylene containing from 1 to 10 carbon atoms,
wherein n and o are integers from 0 to 6, and m is an integer from 1 to 6.
28 . The compound of claim 27 , wherein X is
wherein R 3 is hydrogen or taken together with its attached oxygen atom forms a reactive ester and R 4 is oxygen or sulfur.
29 . An immunogen containing an immunogenic polyamine polymer linked to a compound of the formula:
Y is an organic spacing group;
X is a functional terminal group capable of binding to a polyamine polypeptide; and
p is an integer from 0 to 1.
30 . The compound of claim 29 , wherein p is 0.
31 . The compound of claim 29 , wherein p is 1.
32 . The compound of claim 31 , wherein Y is alkylene containing from 1 to 10 carbon atoms,
wherein n and o are integers from 0 to 6, and m is an integer from 1 to 6.
33 . The compound of claim 32 , wherein the immunogenic polymer contains one or more amino groups linked by
wherein R 4 is oxygen or sulfur.
34 . An immunogen comprising an immunogenic polyamine polymer linked to a compound of the formula:
Y is an organic spacing group;
X is a functional terminal group capable of binding to a polyamine polypeptide; and
p is an integer from 0 to 1.
35 . The compound of claim 34 , wherein p is 0.
36 . The compound of claim 35 , wherein the immunogenic polymer contains one or more amino groups linked by
wherein R 4 is oxygen or sulfur.
37 . The compound of claim 34 , wherein p is 1.
38 . The compound of claim 37 , wherein Y is alkylene containing from 1 to 10 carbon atoms,
wherein n and o are integers from 0 to 6, and m is an integer from 1 to 6.
39 . The compound of claim 38 , wherein the immunogenic polymer contains one or more amino groups linked by
wherein R 4 is oxygen or sulfur.