Nylon binding peptides and methods of use

1. A peptide reagent having a general structure selected from the group consisting of:

a) NY m -(NYBP-BA p ) n ;

b) NY m -(NYBP-AD) n ;

c) NY m -(NYBP-TBD) n ;

d) NY m -(NYBP-L-BA) n ; and

e) NY m -[(NYBP) q -L(x)-(NYBP) r ] n -L-BA;

wherein:

i) NY is a nylon moiety selected from the group consisting of: nylon coated beads, nylon coated print media, and nylon films;

ii) NYBP is a nylon binding peptide having a nylon binding domain comprising an amino acid sequence selected from the group consisting of SEQ ID NOs: 1, 2, 3, 4, 5, and 6;

iii) BA is at least one benefit agent selected from the group consisting of: pharmaceuticals, markers, colorants, conditioners and fragrances;

iv) AD is at least one active domain incorporated into a nylon binding peptide; wherein the at least one active domain has antimicrobial functionality and comprises an amino acid sequence selected from the group consisting of SEQ ID NOs: 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, and 41;

v) TBD is at least one target binding domain incorporated into a nylon binding peptide wherein the target binding domain has affinity for pigments, affinity for benefit agents, affinity for print media, affinity for chemical functional groups, or affinity for biological analytes; wherein the at least one target binding domain comprises an amino acid sequence selected from the group consisting of SEQ ID NOs: 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, and 79;

vi) L is a linker molecule;

vii) m=the number of nylon moieties available for binding;

viii) n=is less than or equal to m;

ix) p=1-20;

x) x=1-20; and

xi) r=1-50.

2. A peptide reagent according to claim 1 wherein the linker molecule is selected from the group consisting of: a peptide linker, and an organic linker.

3. A peptide reagent according to claim 1 wherein the target binding domain is a print media binding domain.

4. A peptide reagent according to claim 1 wherein the target binding domain comprises a pigment binding domain.

5. A peptide reagent according to claim 1 wherein the print medium is selected from the group consisting of paper, sheets, films, nonwovens and textile fabrics.

6. A peptide reagent according to claim 1 , wherein the pharmaceutical is selected from the group consisting of: antibacterial agents, antiviral agents, antifungal agents, anti-cancer agents, vaccines, radiolabels, anti-inflammatories, anti-glaucomic agents, anesthetics, anti-neoplastic agents, antibodies, and hormones.

7. A peptide reagent according to claim 1 , wherein the marker is selected from the group consisting of: a fluorescent label and an enzyme.