Polytetrafluoroethylene binding peptides and methods of use

1. A peptide reagent having a general structure selected from the group consisting of:

a) PTFE m -(PTFEBP-BA p ) n ;

b) PTFE m -(PTFEBP-AD) n ;

c) PTFE m -(PTFEBP-TBD) n ;

d) PTFE m -(PTFEBP-L-BA) n ; and

e) PTFE m -[(PTFEBP) q -L (x) -(PTFEBP) r ] n -L-BA;

wherein:

i) PTFE is a polytetrafluoroethylene moiety non-covalently bound to PTFEBP;

ii) PTFEBP is a PTFE binding peptide selected from the group consisting of SEQ ID NOs: 1-9;

iii) BA is at least one benefit agent covalently or non-covalently bound to PTFEBP selected from the group consisting of pharmaceuticals, markers, colorants, conditioners and fragrances;

iv) AD is at least one active domain incorporated into a PTFE binding peptide, wherein the AD has a functionality selected from the group consisting of a catalytic functionality and an antimicrobial functionality;

v) TBD is at least one target binding domain incorporated into a PTFE binding peptide, wherein the TBD has an affinity selected from the group consisting of affinity for pigments, affinity for print media and affinity for body surfaces;

vi) L is a liniker;

vii) m=the number of PTFE moieties available for binding;

viii) n is less than or equal to m;

xi) p=1-20;

x) x=1-20; and

xi) r=1-50.

2. A peptide reagent according to claim 1 wherein the target binding domain is a body surface binding domain.

3. A peptide reagent according to claim 2 wherein the body surface binding domain binds to a body surface selected from the group consisting of hair, skin, nails and teeth.

4. A peptide reagent according to claim 3 wherein the body surface binding domain binding hair has an amino acid sequence selected from the group consisting of SEQ ID NO's: 88-175.

5. A peptide reagent according to claim 3 wherein the body surface binding domain binding skin has an amino acid sequence selected from the group consisting of SEQ ID NO's: 80-87.

6. A peptide reagent according to claim 3 wherein the body surface binding domain binding nails has an amino acid sequence selected from the group consisting of SEQ ID NO's: 88 and 89.

7. A peptide reagent according to claim 1 wherein the target binding domain is a print media binding domain.

8. A peptide reagent according to claim 7 wherein the print media binding domain has the amino acid sequence selected from the group consisting of SEQ ID NO's: 67-79.

9. A peptide reagent according to claim 1 wherein the target binding domain comprises a pigment binding domain.

10. A peptide reagent according to claim 9 wherein the pigment binding domain has the amino acid sequence selected from the group consisting of SEQ ID NO's: 42-66.

11. A peptide reagent according to claim 1 wherein the active domain comprises an animicrobial domain.

12. A peptide reagent according to claim 11 wherein the antimicrobial domain has an amino acid sequence selected from the group consisting of SEQ ID NO's: 13-41.

13. A peptide reagent according to either claim 1 wherein the PTFE moiety is incorporated into a surface.

14. A peptide reagent according to claim 13 wherein the surface is selected from the group consisting of a solid support, a bead, a microsphere, a sheet, and a fiber.

15. A peptide reagent according to claim 14 wherein the microsphere comprises a dye.

16. A peptide reagent according to claim 13 wherein the surface is coated on secondary surface.

17. A peptide reagent according to either claim 1 wherein the PTFE moiety is comprised within a PTFE film.

18. A peptide reagent according to either of either claim 1 wherein the PTFE moiety is comprised within a print medium.

19. A peptide reagent according to claim 18 wherein the print medium is selected from the group consisting of paper, sheets, films, nonwovens and textile fabrics.

20. A peptide reagent according claim 1 wherein the pharmaceutical is selected from the group consisting of antibacterial agents, antiviral agents, antifungal agents, anti-cancer agents, vaccines, radiolabels, anti-inflammatories, anti-glaucomic agents, anesthetics, anti-neoplastic agents, antibodies, and hormones.

21. A peptide reagent according claim 1 wherein the marker is selected from the group consisting of: a fluorescent label and an enzyme.

22. A peptide reagent according claim 1 wherein the conditioner is a hair conditioner selected from the group consisting of cationic polymers; cationic surfactants, fatty alcohols, fatty amines; waxes; esters; nonionic polymers, silicones; siloxanes; polymer emulsions; and volumizing agents.

23. A peptide reagent according claim 1 wherein the conditioner is a skin conditioner selected from the group consisting of alpha-hydroxy acids, beta-hydroxy acids, polyols, hyaluronic acid, D,L-panthenol, polysalicylates, vitamin A palmitate, vitamin E acetate, glycerin, sorbitol, silicones, silicone derivatives, lanolin, natural oils and triglyceride esters.

24. A peptide reagent according claim 1 wherein the colorant is a hair colorant selected from the group consisting of D&C Yellow 1 and 3, HO Yellow 6 and 8, D&C Blue 1, HO Blue 1, HO Brown 2, HO Red 5,2-nitro-paraphenylenediamine, N,N-hydroxyethyl-2-nitro-phenylenediamine, 4-nitro-indole, iron oxides, and carbon black.

25. A peptide reagent according claim 1 wherein the colorant is a skin colorant selected from the group consisting of D&C Red No. 21; D&C Red No. 27, D&C Red Orange No. 5; D&C Orange No. 10, titanium dioxide, zinc oxide, D&C Red No. 36; D&C Orange No. 17, the calcium lakes of D&C Red Nos. 7, 11, 31 and 34, the barium lake of D&C Red No. 12, the strontium lake D&C Red No. 13, the aluminum lakes of FD&C Yellow No. 5, of FD&C Yellow No. 6, of D&C Red No. 27, of D&C Red No. 21, of FD&C Blue No. 1, iron oxides, manganese violet, chromium oxide, ultramarine blue, and carbon black.

26. A peptide reagent according claim 1 wherein the colorant is a nail colorant selected from the group consisting of D&C Red Nos. 8, 10, 30 and 36, the barium lakes of D&C Red Nos. 6, 9 and 12, the calcium lakes of D&C Red Nos. 7, 11, 31 and 34, the strontium lake of D&C Red No. 30 and D&C Orange No. 17 and D&C Blue No. 6.