Polypropylene binding peptides and methods of use
View Patent ↗Combinatorially generated peptides are provided that have binding affinity for polypropylene (PP). The peptides may be used to deliver benefit agents to various PP surfaces.
1. A peptide reagent having a general structure selected from the group consisting of:
a) PP m -(PPBP-BAp)n; and
b) PP m -(PPBP-L-BA)n;
wherein:
i) PP is a polypropylene moiety
ii) PPBP is a polypropylene binding peptide having a polypropylene binding domain;
iii) BA is at least one benefit agent wherein the at least one benefit agent is a colorant;
iv) L is a linker molecule;
v) m=the number of polypropylene moieties available for binding, and m is at least one;
vi) n=m; and
vii) p=1-20.
2. A peptide reagent according to claim 1 wherein the polypropylene binding peptide comprises a polypropylene binding sequence selected from the group consisting of SEQ ID NO's: 1-7.
3. A peptide reagent according to claim 1 wherein the linker molecule is selected from the group consisting of: a peptide linker, and an organic linker.
4. A peptide reagent according to claim 1 wherein the polypropylene moiety is incorporated into a surface.
5. A peptide reagent according to claim 4 wherein the surface is selected from the group consisting of a solid support, a bead, a microsphere, a sheet, and a fiber.
6. A peptide reagent according to claim 5 wherein the microsphere comprises a dye.
7. A peptide reagent according to claim 4 wherein the surface is coated on a secondary surface.
8. A peptide reagent according to claim 1 wherein the polypropylene moiety is comprised within a polypropylene film.
9. A peptide reagent according to claim 1 wherein the colorant is a hair colorant selected from the group consisting of D&C Yellow 1 and 3, HC Yellow 6 and 8, D&C Blue 1, HC Blue 1, HC Brown 2, HC Red 5, 2-nitro-paraphenylenediamine, N,N-hydroxyethyl-2-nitro-phenylenediamine, 4-nitro-indole, iron oxides, and carbon black.
10. A peptide reagent according to claim 1 wherein the polypropylene binding peptide is isolated by a process comprising the steps of:
(i) providing a library of combinatorially generated peptides;
(ii) contacting the library of (i) with a polypropylene sample to form a reaction solution comprising:
(A) peptide-polypropylene complex;
(B) unbound polypropylene, and
(C) uncomplexed peptides;
(iii) isolating the peptide-polypropylene complex of (ii);
(iv) eluting the weakly bound peptides from the isolated peptide complex of (iii) whereby the polypropylene binding peptide is isolated.