CULTURE CELLS FROM PANCREATIC ISLETS
A cell composition of endocrine progenitor cells derived from mammalian pancreatic islet cells that can be trans-planted planted into a diabetic patient such t the cells of the cell composition differentiates into functioning insulin-producing beta cells.
1 - 3 . (canceled)
4 . A method for implanting cells functioning as insulin-producing beta cells into a patient with diabetes, comprising:
(a) serially culturing cells derived from human mammalian pancreas islet cells in a defined cultured medium to expand the cells and to produce endocrine precursor cells in the absence of fibroblast overgrowth, wherein the defined culture medium comprises amino acids, growth factors, hormones, and lipids in the absence of serum or organ extracts, and wherein the endocrine precursor cells have less differentiation than the initially cultured cells and wherein the endocrine precursor cells express markers pdx1 and nestin; and
(b) implanting the endocrine precursor cells into a recipient patient with diabetes.