System for dissociation and removal of proteinaceous tissue
View Patent ↗An apparatus and method for the dissociation of soft proteinaceous tissue using pulsed rapid variable direction energy field flow fractionization is disclosed. The pulsed rapid disruptive energy field is created by the use of a probe which surrounds the soft proteinaceous tissue to be removed. Once the adhesive mechanism between tissue constituents has been compromised, fluidic techniques are used to remove the dissociated tissue.
1. A method of using a high-intensity pulsed electric field to increase the fluidity of a volume of ocular tissue, the method comprising:
(a) providing a volume of ocular tissue having protein bonds; and
(b) applying a localized pulsed electric field to the ocular tissue without causing an electron avalanche; wherein the localized pulsed electric field is generated by applying to the ocular tissue a series of electric pulses, the series of electric pulses having a pulse duration shorter than a dielectric relaxation time of the protein bonds and having an electric field strength greater than one KV/cm and up to 300 KV/cm; and further wherein the series of electric pulses is delivered to the ocular tissue by two or more electrodes such that said applying of the series of electric pulses increases the fluidity of the ocular tissue by dissociating the protein bonds.
2. The method of claim 1 , further comprising:
changing a shape of a pulse in the series of electric pulses.
3. The method of claim 1 , further comprising:
changing a width of a pulse in the series of electric pulses.
4. The method of claim 1 , further comprising:
changing a rise time of a pulse in the series of electric pulses.
5. The method of claim 1 , further comprising:
changing a fall time of a pulse in the series of electric pulses.
6. The method of claim 1 , further comprising:
changing a pulse repetition rate in the series of electric pulses.
7. The method of claim 1 , further comprising:
changing a number of pulses in the series of electric pulses.
8. The method of claim 1 , further comprising changing a pulse-train repetition-rate in the series of electric pulses.
9. The method of claim 1 , further comprising:
changing a pattern of the pulses in the series of electric pulses.
10. The method of claim 1 , further comprising:
changing an amplitude of a pulse in the series of electric pulses.
11. The method of claim 1 , further comprising:
changing a number of pulse-trains in the series of electric pulses delivered to the ocular tissue.
12. The method of claim 1 , further comprising:
changing a polarity of a pulse in the series of electric pulses.
13. The method of claim 1 , wherein the two or more electrodes are arranged in an array and wherein the series of electric pulses is delivered by providing electrical switching of each electric pulse between the two or more electrodes.
14. The method of claim 1 , wherein the electrical switching changes a number of electrodes which are activated in the array.
15. The method of claim 14 , further comprising:
changing an activation sequence of the active electrodes.
16. The method of claim 1 , further comprising:
changing a direction of the electric field.
17. The method of claim 1 , further comprising:
changing a polarity of the electric field.
18. The method of claim 1 , wherein the pulses have squarish or trapezoidal shape.
19. The method of claim 1 , wherein the pulse duration in the series of electric pulses is in the sub-microsecond range.
20. The method of claim 1 , wherein a pulse-rise-time in the series of electric pulses is shorter than 5 nanoseconds.
21. The method of claim 1 , wherein a pulse-fall-time in the series of electric pulses is shorter than 5 nanoseconds.