Methods for production of proteins
View Patent ↗The current invention provides methods for producing a polypeptide as inclusion bodies in bacterial host cells. The present methods are carried out by forming a gene construct comprising the genetic sequence encoding a polypeptide operatively linked to that of an inclusion partner protein, such as E. coli thioredoxin or a modified E. coli thioredoxin, such that host cells comprising the gene construct produce the polypeptide as intracellular inclusion bodies. The methods of the present invention facilitate the rapid isolation and purification of recombinant proteins. In addition, the present methods may be useful for producing polypeptides or proteins which are small and are typically difficult to express, as well as those proteins that are toxic to host cells such as E. coli . The present invention also provides plasmids, vectors and host cells to be used in the present invention for production of polypeptides, and methods of production of polypeptides using these vectors and host cells. The invention further provides methods for producing protein molecular weight ladders for use in protein gel electrophoresis, as well as proteins and protein molecular weight ladders produced by these methods.
1. A method for making a protein molecular weight ladder composition comprising three or more proteins of differing molecular weights, the method comprising:
(a) obtaining three or more nucleic acid molecules;
wherein each of the three or more nucleic acid molecules encodes a recombinant protein or recombinant protein fragment;
wherein at least one of the three or more nucleic acid molecules comprises multimers of a DNA fragment encoding at least a portion of a protein;
(b) transforming host cells with the nucleic acid molecules;
(c) culturing the host cells under conditions favoring the production of the recombinant proteins or recombinant protein fragments of the molecular weight ladder as inclusion bodies;
(d) isolating each of the recombinant proteins or recombinant protein fragments; and
(e) admixing each of the different recombinant proteins or recombinant protein fragments to form a molecular weight ladder.
2. The method of claim 1 , wherein at least one of the three or more nucleic acid molecules encodes a plurality of the recombinant proteins or recombinant protein fragments of different molecular weights of the molecular weight ladder.
3. The method of claim 1 , wherein the three or more nucleic acid molecules each encode a different recombinant protein or recombinant protein fragment of the molecular weight ladder.
4. The method of claim 1 , wherein the three or more recombinant proteins are separated by at least one molecular weight increment selected from the group consisting of 5 kD, 10 kD, 20 kD, 25 kD, 50 kD, and 100 kD.
5. The method of claim 1 , wherein the nucleic acid molecule is inserted into a vector prior to transforming the host cells.
6. The method of claim 5 , wherein the vector is an expression vector.
7. The method of claim 1 , wherein the recombinant proteins or recombinant protein fragments are contacted with one or more dyes to form a pre-stained molecular weight ladder.
8. The method of claim 1 , wherein the molecular weight ladder comprises three or more recombinant proteins or recombinant protein fragments of different sizes.
9. The method of claim 1 , wherein the molecular weight ladder comprises 3-20 recombinant proteins or recombinant protein fragments of different sizes.
10. The method of claim 1 , wherein the molecular weight ladder comprises 3-15 recombinant proteins or recombinant protein fragments of different sizes.
11. The method of claim 1 , wherein the molecular weight ladder comprises 3-10 recombinant proteins or recombinant protein fragments of different sizes.
12. The method of claim 1 , wherein the molecular weight ladder comprises a collection of recombinant proteins or recombinant protein fragments ranging from 5 kD to 300 kD.
13. The method of claim 1 , wherein the molecular weight ladder comprises a collection of recombinant proteins or recombinant protein fragments ranging from about 5 kD to about 250 kD.
14. The method of claim 1 , wherein the molecular weight ladder comprises a collection of recombinant proteins or recombinant protein fragments ranging from about 10 kD to about 220 kD.
15. The method of claim 1 , wherein the molecular weight ladder comprises a collection of recombinant proteins or recombinant protein fragments in increments of 5 kD, 10 kD, 20 kD, 25 kD, 50 kD, or 100 kD.
16. The method of claim 1 , further comprising altering the length or number of copies of a gene encoding the recombinant proteins or recombinant protein fragments of the one or more nucleic acid molecules to define the molecular weight increments of the ladder.
17. The method of claim 1 , further comprising generating fusion proteins by linking a fragment encoding at least a portion of a first polypeptide to a nucleic acid fragment encoding at least a portion of a second polypeptide.
18. The method of claim 1 , further comprising generating fusion proteins by linking multimers of a fragment encoding at least a portion of a polypeptide.
19. The method of claim 1 , further comprising linking a fragment encoding at least a portion of a first polypeptide to a nucleic acid fragment encoding at least a portion of a peptide tag.
20. The method of claim 19 , further comprising linking a fragment encoding at least a portion of a first polypeptide to a nucleic acid fragment encoding at least a portion of a HIS-tag.
21. The method of claim 1 , further comprising admixing each of the different polypeptides and at least one additional polypeptide to form a molecular weight standard that comprises a ladder.
22. The method of claim 21 , wherein at least one additional polypeptide is a non-recombinant polypeptide.
23. The method of claim 1 , wherein the portion of the gene encoded by multimers of a DNA fragment is a portion of a thioredoxin gene.
24. The method of claim 1 , wherein the molecular weight of at least one of the recombinant proteins or recombinant protein fragments is 10 kD, 15 kD, 20 kD, 25 kD, 30 kD, 40 kD, 50 kD, 60 kD, 70 kD, 80 kD or 100 kD.