Methods and compositions for the production of high concentration alloxazine solutions
View Patent ↗Methods are provided for preparation of compositions having an enhanced level of soluble alloxazine, as compared to compositions prepared using conventional techniques. Compositions and a riboflavin form having higher solubility in solution is also provided.
1. A method of increasing the concentration of an alloxazine in an aqueous medium consisting of sodium chloride to above the alloxazine's saturation point, the method comprising:
adding an amount of said alloxazine to an aqueous medium consisting of about 0.9% sodium chloride at a pH of between about 4 and about 5, wherein the amount of alloxazine exceeds the saturation point of said alloxazine at 1 atmosphere and 22° C:
heating said aqueous medium consisting of sodium chloride and alloxazine to a temperature between about 80° C. and about 90° C; and
cooling said aqueous medium consisting of sodium chloride and alloxazine to produce an aqueous medium having a concentration of alloxazine above the alloxazine saturation point.
2. The method of claim 1 , wherein the alloxazine is riboflavin.
3. The method of claim 1 , further comprising sterilizing the aqueous medium consisting of sodium chloride and alloxazine after cooling under pressure at a temperature of at least 120° C.