Generation of plants with altered oil content
View Patent ↗The present invention is directed to plants that display an altered oil content phenotype due to altered expression of a HIO nucleic acid. The invention is further directed to methods of generating plants with an altered oil content phenotype.
1. A transgenic plant comprising a heterologous polynucleotide that encodes a HIO polypeptide having the amino acid sequence set forth as SEQ ID NO: 2 or an amino acid sequence having at least 95% sequence identity to the amino acid sequence set forth as SEQ ID NO: 2,
whereby the transgenic plant has a high oil phenotype relative to a plant of the same species that does not comprise the heterologous polynucleotide.
2. The transgenic plant of claim 1 , which is selected from the group consisting of rapeseed, soy, corn, sunflower, cotton, cocoa, safflower, oil palm, coconut palm, flax, castor and peanut.
3. A plant part obtained from the plant according to claim 1 .
4. The plant part of claim 3 , which is a seed.
5. Meal, feed, or food produced from the seed of claim 4 .
6. A method of producing oil comprising growing the transgenic plant of claim 1 and recovering oil from said plant.
7. The method of claim 6 , wherein the oil is recovered from a seed of the plant.
8. A method of producing a plant having a high oil phenotype, said method comprising:
a) introducing into progenitor cells of the plant a heterologous polynucleotide that encodes a HIO polypeptide having the amino acid sequence set forth as SEQ ID NO: 2, or an amino acid sequence having at least 95% sequence identity to the amino acid sequence set forth as SEQ ID NO: 2; and
b) growing the transformed progenitor cells to produce a transgenic plant,
wherein said heterologous polynucleotide is expressed, and said transgenic plant exhibits a high oil content phenotype relative to a plant of the same species that does not comprise the heterologous polynucleotide.
9. A plant obtained by a method of claim 8 .
10. The plant of claim 9 , which is selected from the group consisting of rapeseed, soy, corn, sunflower, cotton, cocoa, safflower, oil palm, coconut palm, flax, castor and peanut.
11. A feed, meal, grain, food, or seed comprising a polypeptide encoded by the nucleic acid sequence as set forth in SEQ ID NO:1.
12. A feed, meal, grain, food, or seed comprising a polypeptide comprising an amino acid sequence as set forth in SEQ ID NO:2.
13. The transgenic plant of claim 1 , wherein the heterologous polynucleotide encodes a HIO polypeptide having an amino acid sequence having at least 95% sequence identity to the amino acid sequence of SEQ ID NO: 2.
14. The transgenic plant of claim 13 , wherein the heterologous polynucleotide encodes a HIO polypeptide having the amino acid sequence set forth as SEQ ID NO: 2.
15. The transgenic plant of claim 14 , wherein the heterologous polynucleotide encodes a HIO polypeptide consisting of the amino acid sequence set forth as SEQ ID NO: 2.
16. The method claim 8 , wherein the heterologous polynucleotide encodes a HIO polypeptide having an amino acid sequence having at least 95% sequence identity to the amino acid sequence of SEQ ID NO: 2.
17. The method of claim 16 , wherein the heterologous polynucleotide encodes a HIO polypeptide having the amino acid sequence set forth as SEQ ID NO: 2.
18. The method of claim 17 , wherein the heterologous polynucleotide encodes a HIO polypeptide consisting of the amino acid sequence set forth as SEQ ID NO: 2.